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METHOD FOR DETERMINING EFFECTIVENESS OF MEDICINE CONTAINING ANTIBODY AS COMPONENT

Foreign code F120006997
File No. S2010-1131-C0
Posted date Oct 29, 2012
Country WIPO
International application number 2011JP004763
International publication number WO 2012/035705
Date of international filing Aug 26, 2011
Date of international publication Mar 22, 2012
Priority data
  • P2010-209322 (Sep 17, 2010) JP
Title METHOD FOR DETERMINING EFFECTIVENESS OF MEDICINE CONTAINING ANTIBODY AS COMPONENT
Abstract

Provided is a method for determining effectiveness of medicine containing, as components, antibodies that are active components of an antibody preparation such as trastuzumab, or antibodies for a target to a target site of active component, by determining the quantity of the protein recognized by the antibodies with high accuracy, in a quantitative tissue staining method for biological tissue. The effectiveness of the medicine containing antibodies as components is determined by using the tissue staining method including the following steps: a step of labeling the antibodies in a medicine that contains antibodies as components with a fluorescent substance, and bringing the fluorescently labeled antibodies into contact with a tissue sample

a step of irradiating the tissue site contacted with the antibodies with excitation light to obtain a fluorescence image

a step of obtaining an intrinsic fluorescence image in the vicinity of the short-wavelength side or long-wavelength side of the acquisition area of fluorescence wavelength emitted from the fluorescent substance, in the same field of vision and in the same focus as those of the fluorescence image

a step of obtaining a collected fluorescence image by image-processing to eliminate the fluorescent intensity of the intrinsic fluorescence image from the fluorescent intensity of the fluorescence image

a step of counting the number of cells at the tissue site contacted with the antibodies

a step of measuring the average fluorescent intensity of one particle of fluorescent particles

and a step of calculating the number of fluorescent particles per cell.

  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • TOHOKU UNIVERSITY,
  • MIYASHITA, MINORU,
  • GONDA, KOHSUKE,
  • OHUCHI, NORIAKI,
  • TAKEDA, MOTOHIRO
  • Inventor
  • MIYASHITA, MINORU,
  • GONDA, KOHSUKE,
  • OHUCHI, NORIAKI,
  • TAKEDA, MOTOHIRO
IPC(International Patent Classification)
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