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SUBSTRATE FOR STEM CELL CULTURE, AND CULTURE METHOD USING SAME

外国特許コード F130007118
整理番号 S2011-0831-N0
掲載日 2013年1月17日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2012JP066491
国際公開番号 WO 2013002311
国際出願日 平成24年6月28日(2012.6.28)
国際公開日 平成25年1月3日(2013.1.3)
優先権データ
  • 特願2011-142403 (2011.6.28) JP
発明の名称 (英語) SUBSTRATE FOR STEM CELL CULTURE, AND CULTURE METHOD USING SAME
発明の概要(英語) Provided are a substrate for stem cell cultures having no risk of pathogen infection or undesirable side effects, and a method for using the same. The present invention comprises a substrate for stem cell cultures in which a cell adhesion peptide having a cyclic skeleton structure is bonded to a polymer substrate, and a stem cell culture method using the substrate for stem cell cultures. A substance containing the Arg-Gly-Asp sequence found in fibronectin can be used as the cell adhesion peptide with a cyclic skeleton structure. A polypeptide and/or a polysaccharide can be used for the polymer substrate.
従来技術、競合技術の概要(英語) BACKGROUND ART
Embryonic stem cells (ES cells) or induced pluripotent stem cells such as stem cells (iPS cells), tissue and organ reconstruction and inside and outside of the living body, instead of the lost tissue and organ transplantation in order to realize the essential for regenerative medicine. The current, while maintaining the pluripotent stem cells for expansion, an animal or human-derived fibroblasts should be used as feeder cells, animal-derived materials such as gelatin and matrigel coated culture vessel is used. These techniques are, or an animal an animal or human fibroblasts from prion-derived material or unknown viruses and other pathogens is at risk of contamination, and prevents achievement of regenerative medicine is one of the factors.
The inventors of the invention, an animal or human-derived material and an alternative to a high level of safety in the creation of a biocompatible material with the aim of earnest studies, which have such properties are provided in a full chemical synthesis of polypeptide synthesis techniques are completed. For example, in patent document 1, pathogen infection or a risk of transmission of the pathogenic agent and which may produce undesirable side effects, such as various physiologically active substances or apatite as a novel polypeptide useful as the carrier of the, - Pro-X-Gly - (X is, represents Pro or Hyp) having the amino acid sequence represented by and cleaved, is -Pro-Hyp(O-Y-Z) -Gly-(Y, carbonyl group, does not have a carbonyl group or a saturated or unsaturated hydrocarbon group, or an aromatic group, a carbonyl group or saturated or unsaturated does not represent a hydrocarbon group, Z represents a carboxyl group) having the amino acid sequence represented by cleaved and a polypeptide comprising the disclosed.
On the other hand, the non-patent document 2, found in fibronectin cell adhesion sequence Gly-Arg-Gly-Asp-Ser and poly Pro-His-Ser-Arg-Asn(Pro-Hyp-Gly) polypeptide materials attached to a mouse fibroblast cell line is the cell adhesion of NIH3T3 and facilitating movement of, and rabbit corneal epithelial cells to promote layered strains have been reported.
Patent Document 2 is, in the culture environment , while retaining differentiation pluripotency in order to maintain a human pluripotent stem cell, a fragment of the coated human pluripotent stem cell culture base material using this culture method has been proposed.
Patent Document 3 is, from the embryonic stem cells to induce the differentiation of hepatocytes efficiently to provide a method and a material thereof for the purpose, as a main component of the crosslinking polysaccharide sponge form of embryonic stem cells culture base material has been proposed.
Is Patent Document 4, feeder cells and feeder cell-derived component in the absence, quantities and safely maintain undifferentiated embryonic stem cells in the culture substrate and cultured for the purpose to provide a method, a porous body made of a nonwoven fabric or the like has been proposed from the culture substrate.
Patent Document 5 is, stem cells, while maintaining its differentiation efficiently to provide a culture substrate for growing the purpose, a substance with a cell adhesion activity characterized in that the stem cell culture base material has been proposed.
Is the non-patent document 3, a cell adhesion sequence Arg-Gly-Asp, the surface of the polymer containing polylactic acid using the same method of combining a method for constructing the bone tissue is reported.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • NATIONAL UNIVERSITY CORPORATION NARA INSTITUTE OF SCIENCE AND TECHNOLOGY
  • 発明者(英語)
  • TANIHARA, Masao
  • SATO, Narutoku
  • KOTOKU, Tomomi
  • SHIBASAKI, Yoshiaki
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IS JP KE KG KM KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PE PG PH PL PT QA RO RS RU RW SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG
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