METHOD FOR FABRICATING STABLE-ISOTOPE-LABELED TARGET PEPTIDE FRAGMENT IN MASS SPECTROMETRY
The present invention addresses the problem of providing a method for fabricating a stable-isotope-labeled target peptide fragment in mass spectrometry, capable of low-cost and simple fabrication. As a solution, a stable-isotope-labeled target peptide fragment in mass spectrometry is fabricated using a method comprising: a step for preparing a stable-isotope-labeled protein by causing a conjugate DNA, which is obtained between tandem-coupled DNA in which two or more DNA has been coupled in tandem for coding two or more species of target peptide fragments and DNA for coding peptide fragments for concentration measurement, to be expressed in a system in which a stable-isotope-labeled amino acid is present
a step for fragmentizing stable-isotope-labeled protein using trypsin to prepare stable-isotope-labeled peptide fragments for concentration measurement and stable-isotope-labeled target peptide fragments
a step for determining the quantity of stable-isotope-labeled peptide fragments for concentration measurement using a liquid chromatography-tandem mass spectrometry device (LC/MS/MS)
and a step for calculating the concentration of stable-isotope-labeled target peptide fragments from the quantified value of the stable-isotope-labeled peptide fragments for concentration measurement.