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HETEROLOGOUS EXPRESSION OF FUNGAL POLYKETIDE SYNTHETIC GENE IN YEAST meetings

Foreign code F130007193
Posted date Mar 5, 2013
Country WIPO
International application number 2011JP004566
International publication number WO 2012/020574
Date of international filing Aug 11, 2011
Date of international publication Feb 16, 2012
Priority data
  • P2011-007312 (Jan 17, 2011) JP
  • P2010-181279 (Aug 13, 2010) JP
Title HETEROLOGOUS EXPRESSION OF FUNGAL POLYKETIDE SYNTHETIC GENE IN YEAST meetings
Abstract

Provided is a method which comprises, from a gene containing an eukaryotic intron, removing the intron contained in the gene and ligating exon sequences alone to give an expression vector carrying the thus ligated sequences. Specifically provided are: a method which comprises, from a giant fungal gene containing an intron, amplifying exon sequences, as one or more fragments, by PCR, and ligating the fragments to a vector, which has been treated with a restriction enzyme, by using the gap-repair-cloning method to thereby give an expression vector containing the ligated exon sequences

a method which comprises synthesizing cDNA fragments of a giant gene and ligating the fragments to give an expression vector containing a full-length cDNA sequence

a transformant having an expression vector, which is produced by the aforesaid method, transferred therein

a protein produced by the aforesaid transformant

and a method for obtaining a compound, which is produced by the aforesaid protein, using the aforesaid expression vector.

  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • SHIZUOKA PREFECTURE PUBLIC UNIVERSITY CORPORATION,
  • NATIONAL UNIVERSITY CORPORATION OKAYAMA UNIVERSITY,
  • WATANABE, KENJI,
  • MORIYA, HISAO
  • Inventor
  • WATANABE, KENJI,
  • MORIYA, HISAO
IPC(International Patent Classification)

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