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PROTEIN PRODUCTION METHOD USING TRANSFORMED PLANT CELLS

Foreign code F130007223
File No. S2011-1109-N0
Posted date Mar 22, 2013
Country WIPO
International application number 2012JP071815
International publication number WO 2013/031821
Date of international filing Aug 29, 2012
Date of international publication Mar 7, 2013
Priority data
  • P2011-191180 (Sep 2, 2011) JP
Title PROTEIN PRODUCTION METHOD USING TRANSFORMED PLANT CELLS
Abstract

The purpose of the present invention is to produce a protein by efficiently culturing plant cells while preventing the inhibition of translation in mRNA of the plant cells that are cultured, in particular, under stress conditions due to the lack of a factor essentially required for the growth (for example, nutrient-starvation stress or anoxic stress). Provided are a method, said method comprising a step for culturing plant cells, which have been transformed by a recombinant DNA molecule encoding mRNA having a 5'UTR(a) or (b) as mentioned below, under stress conditions due to the lack of a factor essentially required for the growth, to thereby prevent the inhibition of translation of a protein encoded by the aforesaid mRNA by the stress due to the lack of the factor essentially required for the growth, and so on: (a) a 5'UTR comprising a base sequence represented by SEQ ID NO:1, 2, 3, 4, 5 or 6

and (b) a 5'UTR comprising a base sequence, which is derived from the base sequence of 5'UTR(a) by substitution, deletion or addition of one to several bases, and being capable of preventing the inhibition of translation by the stress due to the lack of the factor essentially required for the growth.

  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • NATIONAL UNIVERSITY CORPORATION NARA INSTITUTE OFSCIENCE AND TECHNOLOGY,
  • KATO, KO,
  • UEDA, KIYOTAKA,
  • OKAWARA, RENYA,
  • YAMURA, TOSHIHIRO
  • Inventor
  • KATO, KO,
  • UEDA, KIYOTAKA,
  • OKAWARA, RENYA,
  • YAMURA, TOSHIHIRO
IPC(International Patent Classification)
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