ELECTRON-MICROSCOPIC EXAMINATION METHOD FOR EXAMINING BIOSAMPLE WHILE KEEPING SAID BIOSAMPLE UNCHANGED, AND COMPOSITION FOR EVAPORATION INHIBITION UNDER VACUUM, SCANNING ELECTRON MICROSCOPE, AND TRANSMISSION ELECTRON MICROSCOPE FOR USE IN SAID METHOD

• 特願2011-197685 (2011.9.9) JP
• 特願2012-044383 (2012.2.29) JP

A method for electron-microscopic examination is provided with which it is possible to examine a biosample with an electron microscope while keeping the biosample alive and to observe the biosample being moving. Also provided are a composition for evaporation inhibition under vacuum, a scanning electron microscope, and a transmission electron microscope which are for use in the method. This method for sample examination by electron microscopy is characterized by comprising: a step in which a composition for evaporation inhibition that comprises at least one ingredient selected from amphipathic compounds, oils and fats, and ionic liquids is applied to the surface of a sample to form a thin film and thus cover the sample with the thin film

and a step in which an electron-microscopic image of the thin-film-covered sample that has been placed in a vacuum sample chamber is displayed in a display device.

• JAPAN SCIENCE AND TECHNOLOGY AGENCY,
• HARIYAMA TAKAHIKO,
• TAKAKU YASUHARU,
• SUZUKI HIROSHI,
• MURANAKA YOSHINORI,
• OHTA ISAO,
• SHIMOMURA MASATSUGU,
• ISHII DAISUKE

• HARIYAMA TAKAHIKO,
• TAKAKU YASUHARU,
• SUZUKI HIROSHI,
• MURANAKA YOSHINORI,
• OHTA ISAO,
• SHIMOMURA MASATSUGU,
• ISHII DAISUKE

• H01J  37/20      物体または材料を支持しまたは位置づける手段；支持体に関連した隔膜壁またはレンズを調節する手段
• G01N   1/28      調査用標本の調製
• G01N  23/225     電子またはイオンマイクロプローブを用いるもの
• H01J  37/18      真空封止