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LINEAR AMPLIFICATION METHOD FOR TRACE DNA FRAGMENT

Foreign code F130007301
File No. S2011-1107-N0
Posted date Apr 17, 2013
Country WIPO
International application number 2012JP074927
International publication number WO 2013/047677
Date of international filing Sep 27, 2012
Date of international publication Apr 4, 2013
Priority data
  • P2011-215318 (Sep 29, 2011) JP
Title LINEAR AMPLIFICATION METHOD FOR TRACE DNA FRAGMENT
Abstract

The amplification by an LM-PCR method has such a problem that amplification accuracy is not satisfactory although amplification sensitivity is high, and the amplification by a T7-IVT method has such a problem that amplification sensitivity is not satisfactory although amplification accuracy is high. The present invention provides a nucleic acid amplification method whereby it becomes possible to solve the problems of these conventional methods. A nucleic acid amplification method comprising the following step (a) and/or step (b): (a) a step of carrying out an enzymatic reaction of a terminal nucleotide transferase in the presence of DNA which cannot be added with a base at the 3'-terminal thereof with the terminal nucleotide transferase

and (b) a step of carrying out the enzymatic reaction of a Klenow enzyme in the co-presence of a poly(A-T7) oligo-DNA primer and the Klenow enzyme and in the presence of DNA that cannot undergo the formation of any double strand in a T7 region of the poly(A-T7) oligo-DNA primer.

  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • PUBLIC UNIVERSITY CORPORATION YOKOHAMA CITY UNIVERSITY
  • Inventor
  • TANIGUCHI, HIDEKI,
  • UENO, YASUHARU
IPC(International Patent Classification)
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