LINEAR AMPLIFICATION METHOD FOR TRACE DNA FRAGMENT
|Posted date||Apr 17, 2013|
|International application number||2012JP074927|
|International publication number||WO 2013/047677|
|Date of international filing||Sep 27, 2012|
|Date of international publication||Apr 4, 2013|
|Title||LINEAR AMPLIFICATION METHOD FOR TRACE DNA FRAGMENT|
The amplification by an LM-PCR method has such a problem that amplification accuracy is not satisfactory although amplification sensitivity is high, and the amplification by a T7-IVT method has such a problem that amplification sensitivity is not satisfactory although amplification accuracy is high. The present invention provides a nucleic acid amplification method whereby it becomes possible to solve the problems of these conventional methods. A nucleic acid amplification method comprising the following step (a) and/or step (b): (a) a step of carrying out an enzymatic reaction of a terminal nucleotide transferase in the presence of DNA which cannot be added with a base at the 3'-terminal thereof with the terminal nucleotide transferase
and (b) a step of carrying out the enzymatic reaction of a Klenow enzyme in the co-presence of a poly(A-T7) oligo-DNA primer and the Klenow enzyme and in the presence of DNA that cannot undergo the formation of any double strand in a T7 region of the poly(A-T7) oligo-DNA primer.
|IPC(International Patent Classification)|
Contact Information for " LINEAR AMPLIFICATION METHOD FOR TRACE DNA FRAGMENT "
- Public University Corporation Yokohama City University Research Promotion Division
- URL: https://www.yokohama-cu.ac.jp/
- Address: 22-2, Seto, Kanazawa-ku, Yokohama, Japan , 236-0027
- Phone: 81-45-787-2442
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