Top > Search of International Patents > ANTIBODY REACTING WITH NATIVE COCHLIN-TOMOPROTEIN (CTP) AND METHOD FOR MEASURING CTP USING SAME

ANTIBODY REACTING WITH NATIVE COCHLIN-TOMOPROTEIN (CTP) AND METHOD FOR MEASURING CTP USING SAME

Foreign code F130007314
File No. S2012-1237-N0
Posted date Apr 26, 2013
Country WIPO
International application number 2012JP058988
International publication number WO 2012133898
Date of international filing Apr 2, 2012
Date of international publication Oct 4, 2012
Priority data
  • P2011-080052 (Mar 31, 2011) JP
Title ANTIBODY REACTING WITH NATIVE COCHLIN-TOMOPROTEIN (CTP) AND METHOD FOR MEASURING CTP USING SAME
Abstract Immunological measurement is carried out using an anti-CTP antibody, characterized by recognizing an epitope contained in a polypeptide represented by amino acid numbers 118 to 132 in SEQ ID NO: 1 and reacting with native Cochlin-tomoprotein (CTP).
Outline of related art and contending technology BACKGROUND ART
Or less Cochlin-tomoprotein(, referred to as CTP) is, present in the perilymph of the isoform 16kDa is the molecular weight of Cochlin, cerebrospinal fluid, blood, saliva and the like can be present in the middle ear not found other fluids include, the perilymph fistula has been expected as a diagnostic marker (patent document 1, non-patent document 1-5). Is a perilymph fistula, and the inner ear is spaced from the window opening a hole in the inner ear, middle ear perilymph satisfying the inner ear to leak out of the inner ear dysfunction in exhibit disease. Of the inner ear to early closing the window in order to improve the symptoms, diagnosis at an early stage is very important.
The present inventor's research group, so far, in order to measure the CTP in the biological sample, a synthetic peptide as an immunogen one rabbit polyclonal antibody 4 (Patent Document 1) was prepared. However, that all antibodies reacted only with modified CTP, antibodies reactive with native CTP has not yet been obtained. Therefore, in non-patent document 1-5, the resulting modified CTP-recognizing anti-LCCL3 antibody (not respond to the native CTP) using, in a biological sample by a western blot CTP is being measured.
Scope of claims (In Japanese)請求の範囲 [請求項1]
配列番号1のアミノ酸番号118~132で表されるポリペプチドに含まれる抗原決定基を認識し、未変性Cochlin-tomoprotein(CTP)に反応することを特徴とする抗体。

[請求項2]
配列番号1のアミノ酸番号118~132からなるペプチドを免疫原として得られる、請求項1に記載の抗CTP抗体。

[請求項3]
未変性CTPに反応する第1の抗体で捕捉した未変性のCTPを用意し、該未変性のCTPを認識する第2の抗体を選別する工程を含む、未変性のCTPに反応する抗体のスクリーニング方法。

[請求項4]
前記第1の抗体が請求項1記載の抗体である、請求項3に記載の未変性のCTPに反応する抗体のスクリーニング方法。

[請求項5]
請求項1または2に記載の抗体を少なくとも一つ使用する、配列番号1のアミノ酸番号118~132で表されるポリペプチドを含む蛋白質の免疫学的測定方法。

[請求項6]
配列番号1のアミノ酸番号118~132で表されるポリペプチドを含む蛋白質がCTPである、請求項5に記載の方法。

[請求項7]
請求項1または2に記載の抗体を少なくとも一つ使用してCTPを免疫学的に測定する工程を含む、外リンパ瘻の検査方法。

[請求項8]
請求項1または2に記載の抗体を少なくとも一つ含む、CTP測定キット。

[請求項9]
外リンパ瘻の診断のための請求項8に記載の測定キット。

  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • SAITAMA MEDICAL UNIVERSITY
  • Inventor
  • IKEZONO, Tetsuo
  • SHIKAZE, Satomi
IPC(International Patent Classification)
Specified countries National States: AE AG AL AM AO AT AU AZ BA BB BG BH BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IS JP KE KG KM KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PE PG PH PL PT QA RO RS RU RW SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ MD RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

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