A METHOD FOR NUCLEIC ACID AMPLIFICATION AND APPLICATION THEREOF
This method for nucleic acid amplification is characterized by: having a reaction solution preparation step for preparing a reaction solution containing (a) to (e) below
having a nucleic acid synthesis step for synthesizing nucleic acids using the resultant reaction solution
and not having a nucleic acid thermal denaturation step after the nucleic acid synthesis step. Said method for nucleic acid amplification does not require any special equipment, allows for quick, easy, and low-cost implementation, and has both high amplification efficiency and a high specificity. The reaction solution contains (a) template nucleic acid having repeat sequences, (b) a primer (1) that binds the repeat sequences of the template nucleic acid, (c) a primer (2) that binds the repeat sequences of complementary strand of the template nucleic acid, (d) strand displacement type DNA synthtase, and (e) nucleotides that serves as substrates for the strand displacement type DNA synthetase.