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A METHOD FOR NUCLEIC ACID AMPLIFICATION AND APPLICATION THEREOF meetings

Foreign code F130007337
File No. S2011-0543-C0
Posted date Apr 26, 2013
Country WIPO
International application number 2012JP056381
International publication number WO 2012/124681
Date of international filing Mar 13, 2012
Date of international publication Sep 20, 2012
Priority data
  • P2011-055327 (Mar 14, 2011) JP
Title A METHOD FOR NUCLEIC ACID AMPLIFICATION AND APPLICATION THEREOF meetings
Abstract

This method for nucleic acid amplification is characterized by: having a reaction solution preparation step for preparing a reaction solution containing (a) to (e) below

having a nucleic acid synthesis step for synthesizing nucleic acids using the resultant reaction solution

and not having a nucleic acid thermal denaturation step after the nucleic acid synthesis step. Said method for nucleic acid amplification does not require any special equipment, allows for quick, easy, and low-cost implementation, and has both high amplification efficiency and a high specificity. The reaction solution contains (a) template nucleic acid having repeat sequences, (b) a primer (1) that binds the repeat sequences of the template nucleic acid, (c) a primer (2) that binds the repeat sequences of complementary strand of the template nucleic acid, (d) strand displacement type DNA synthtase, and (e) nucleotides that serves as substrates for the strand displacement type DNA synthetase.

  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY,
  • SUZUKI, YASUHIKO,
  • NAKAJIMA, CHIE,
  • FUKUSHIMA, YUKARI
  • Inventor
  • SUZUKI, YASUHIKO,
  • NAKAJIMA, CHIE,
  • FUKUSHIMA, YUKARI
IPC(International Patent Classification)
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