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NOVEL PEPTIDE COMPLEX AND HYBRID COMPLEX THEREOF, AND USE OF SAID HYBRID COMPLEX

外国特許コード F130007366
整理番号 S2012-0062-N0
掲載日 2013年5月15日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2012JP076654
国際公開番号 WO 2013061818
国際出願日 平成24年10月16日(2012.10.16)
国際公開日 平成25年5月2日(2013.5.2)
優先権データ
  • 特願2011-233812 (2011.10.25) JP
発明の名称 (英語) NOVEL PEPTIDE COMPLEX AND HYBRID COMPLEX THEREOF, AND USE OF SAID HYBRID COMPLEX
発明の概要(英語) The present invention provides a means for delivering a substance of interest into a cell, which, even when the substance of interest is a protein, can introduce the protein into a cell with high efficiency regardless of the type of the protein, enables a given function of the protein to be exerted without inhibiting the function after the introduction of the protein into the cell, and does not use any metal that may affect a living body. A peptide complex [A] according to the present invention is characterized by comprising a leucine zipper peptide [LZ(K)] comprising the amino acid sequence represented by SEQ ID NO: 1: N'-EYQALKKKVAQLKAKNQALKKKVAQLKHK-C' or the like or a leucine zipper peptide [LZ(E)] comprising the amino acid sequence represented by SEQ ID NO: 2: N'-EYQALEKEVAQLEAENQALEKEVAQLEHE-C' or the like and an intracellular invasion peptide [CPP] linked to the leucine zipper peptide.
従来技術、競合技術の概要(英語) BACKGROUND ART
Conventional, the desired protein as a technique for carrying into a cell, the cell entry peptide (cell-penetrating peptide protein; CPP) introducing technologies are the most commonly widely used.
However, the techniques described above CPP fusion protein is used, a protein of interest CPP are connected is vary significantly depending on the type of the production efficiency, can be made not most. In addition, CPP fusion protein in the cell after the introduction, the CPP is sometimes inhibited the function of the protein (CPP is generally positively charged and in many cases, this is the negatively charged nucleic acid or a biological material that interacts with the adversely affected by the possibility has been created).
Such as a technique to deal with the problem, Dowdy et al.'s group is non-patent document 1, histidine 6 to a protein of interest is introduced into the (so-called histidine tag), this modified metal ligands of the metal (nickel) via CPP (without using a covalent bond) by linking the, there is a method of intracellular delivery and confer the ability to described.
Non-patent document 1 is described in the art, are widely used in protein purification generally histidine tag and confer the ability to transport into a cell is used as is, by the use of a metal, a full-fledged for the treatment of intracellular transportation technique, so-called drug delivery system (DDS) in view of application to the anxiety remains.
On the other hand, non-patent document 2-5 Bosshard et al.'s group is, on the basis of the naturally-occurring Fos artificially developed, the mutant-type Fos is disclosed. Is a leucine zipper (coiled coil), a transcription factor DNA-binding motifs found in the dimerization domain of in. To the direction of the dimer peptide 7 amino acid residues and by the repetition of the α helix is included, the position of the 4 th leucine (d) that are arranged, a hydrophobic amino acid residue of a dimer by interaction of a characteristic of the formation of aggregates. Non-patent document disclosed variants Fos, at a predetermined position in the amino acid sequence of amino acids and basic or acidic amino acids (glutamic acid) (lysine) designed so as to include, the natural Fos binding stability than with being improved.
However, disclosed in non-patent document 2-5 mutants Fos, Fos thermodynamic behavior for the purpose of analyzing only those fabricated. On the other hand the Fos dimer linking the peptide to the cell entry and, by utilizing such Fos (such as peptides) of the desired material efficiently can be introduced into cells and the like are to be expressed, not describe or suggest.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • NATIONAL UNIVERSITY CORPORATION OKAYAMA UNIVERSITY
  • 発明者(英語)
  • KITAMATSU, Mizuki
  • MICHIUE, Hiroyuki
  • WANG, Feifei
  • NAKASHIMA, Mami
  • OHTSUKI, Takashi
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IS JP KE KG KM KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

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