TOP > 外国特許検索 > METHOD FOR INDUCING PROTEOLYSIS IN EUKARYOTIC CELL

METHOD FOR INDUCING PROTEOLYSIS IN EUKARYOTIC CELL UPDATE

外国特許コード F130007381
整理番号 S2012-0021-N0
掲載日 2013年6月5日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2012JP079744
国際公開番号 WO 2013073653
国際出願日 平成24年11月16日(2012.11.16)
国際公開日 平成25年5月23日(2013.5.23)
優先権データ
  • 特願2011-252147 (2011.11.18) JP
発明の名称 (英語) METHOD FOR INDUCING PROTEOLYSIS IN EUKARYOTIC CELL UPDATE
発明の概要(英語) Provided are: a novel protein-inducible eukaryotic cell which does not kill a host such as budding yeast and has a satisfactory decomposition ability; and a method for inducing proteolysis using the protein-inducible eukaryotic cell. A proteolysis-inducible non-plant eukaryotic cell, which has a plant-derived TIR1 family protein gene and a chimeric gene capable of expressing a desired protein labeled with a partial sequence of a plant-derived Aux/IAA family protein, and in which the degradation of the desired protein can be induced by an auxin, said non-plant eukaryotic cell being characterized in that the partial sequence is a sequence comprising a region containing at least two Lys residues on each of the N-terminal side and the C-terminal side of a domain II region of an Aux/IAA family protein or a sequence composed of a linked product of two or more of the aforementioned sequences.
従来技術、競合技術の概要(英語) BACKGROUND ART
Specific cells control the expression level of the protein, the function of the protein in the cell and its protein involved in analyzing a biological phenomenon on the very useful. For this purpose, a plurality of separately to based on the principle of this method has been developed.
For example, E. coli using tetracycline-binding transcriptional repressors, transcription of a gene are introduced into the cell culture expression of tetracycline has been known a method of making the (non-patent document 1, 2). Based on this principle are commercially available and transfer system will, many researchers have actually used results as hereinbefore has been increased. However, this method is a method in which the transcriptional control of the gene of interest for, the actual protein suppression may be performed until it is removed from within the cell often takes a long time, in some cases, a partial expression of the protein of interest is the reduction of secondary cellular responses that activate is also sometimes. Suppressing expression in cells cultured similarly in a method of performing siRNA and shRNA to be introduced into a cell, utilizing the reaction with the interference RNA in a cell of the gene product of interest is known to decompose mRNA. This method based on the actual product commercially available from a number of companies. However, this method also mRNA level for suppression of expression of the is carried out, generally to inhibiting the expression of 24-48 requires a long time to time. The expression inhibition degree, independent of the sequence of the target RNA and the target factors, can be realized by suppressing expression of 90% or more is not so high.
In recent years, inducing the target protein is introduced into the tag, the tag can be controlled by controlling the decomposition of a method for controlling the expression of the protein has been begun to be put into practical use. FKBP12 Protein is modified rapamycin binding protein, rapamycin analog compound Shield 1 to bind to the stabilized, is not coupled destabilization from being degraded within the cells. Using this property, this target protein is fuzed to the protein derived from FKBP12, Shield 1-dependent expression control method in which a (non-patent document 3) published. Shield 1 The methods generally involve C. and about 4 by the removal of the target protein and is resolved, and the like are the following commercially available plasmid set required. In this method in order to stabilize the protein expression is always Shield 1 needs to be added to the medium, the rapamycin analog is Shield 1 for the cytotoxic compound is in doubt. In addition, also necessary for suppressing the expression for about 4 in terms of degradation time, the more rapid cell cycle studies such as the systems which can be decomposed and removed is have been desired.
The inventor considers such circumstances, the plant hormone auxin induced by focusing on plant-specific proteolysis, this degradation pathway for the budding yeast and introduced into a cell or mammalian, auxin can be added to the medium a very short time 15-30 min the target protein expression by the decomposition of the developed a method of inhibiting (Patent Document 1, 2).
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • RESEARCH ORGANIZATION OF INFORMATION AND SYSTEMS
  • OSAKA UNIVERSITY
  • 発明者(英語)
  • KANEMAKI, Masato
  • NISHIMURA, Kohei
  • TAKISAWA, Haruhiko
  • MIMURA, Satoru
  • KOBATA, Yui
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IS JP KE KG KM KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

PAGE TOP

close
close
close
close
close
close