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Alga in which production of photosynthetic products is improved, and use for said alga meetings

Foreign code F130007432
File No. AF21-01WO
Posted date Jun 27, 2013
Country United States of America
Application number 201113817360
Gazette No. 20130143260
Gazette No. 9051575
Date of filing Aug 29, 2011
Gazette Date Jun 6, 2013
Gazette Date Jun 9, 2015
International application number JP2011069501
International publication number WO2012029727
Date of international filing Aug 29, 2011
Date of international publication Mar 8, 2012
Priority data
  • P2010-194210 (Aug 31, 2010) JP
  • 2011WO-JP69501 (Aug 29, 2011) WO
Title Alga in which production of photosynthetic products is improved, and use for said alga meetings
Abstract (US9051575)
Provided are an alga in which productivity of a photosynthate is increased, and use of the alga.
The alga of the present invention has an increased glutathione concentration in its chloroplast.
A method of the present invention for producing biomass is a method for producing biomass with the use of the alga of the present invention or an alga produced by a method of the present invention for producing an alga.
Scope of claims [claim1]
1. A method for producing an alga having an increased glutathione concentration in a chloroplast thereof, comprising the steps of: introducing, into an alga, a polynucleotide encoding a gamma -glutamylcysteine synthetase;
cultivating the modified alga into which the polynucleotide has been introduced; and
carrying out screening for the alga having the increased glutathione concentration in the chloroplast, by
(a) measuring an expression amount and/or an activity of an exogenous protein encoded by the polynucleotide and comparing the expression amount and/or the activity thus measured with an expression amount and/or an activity of an endogenous protein in a wild-type alga of a same species; or
(b) measuring the glutathione concentration in the chloroplast obtained from modified alga and comparing the glutathione concentration thus measured with the glutathione concentration in the chloroplast of a wild-type alga of the same species cultivated under same conditions.
[claim2]
2. The method according to claim 1, wherein the polynucleotide encoding the gamma -glutamylcysteine synthetase is selected from the group consisting of the following (a) to (d): (a) a polynucleotide encoding a polypeptide consisting of the amino-acid sequence set forth in SEQ ID NO: 1;
(b) a polynucleotide encoding a polypeptide which consists of an amino-acid sequence with deletion, substitution, or addition of not more than 10 amino acids in the amino-acid sequence set forth in SEQ ID NO: 1 and which has a gamma -glutamylcysteine synthetase activity;
(c) a polynucleotide consisting of the nucleotide sequence set forth in SEQ ID NO: 2; and
(d) a polynucleotide which hybridizes under a stringent condition with a polynucleotide consisting of a nucleotide sequence fully complementary to any one of the polynucleotides (a) through (c) and which encodes a polypeptide having a gamma -glutamylcysteine synthetase activity,
wherein said condition is defined by incubation overnight at 42 deg. C. in 5 * SSC and then washing in 0.1 * SSC at approximately 65 deg. C.
[claim3]
3. A method for producing biomass, comprising the step of cultivating an alga produced by the method as set forth in claim 1.
[claim4]
4. The method according to claim 3, further comprising the step of irradiating the alga with light.
[claim5]
5. The method according to claim 4, wherein the step of irradiating the alga with light is carried out under a condition where nitrogen is not substantially starved.
[claim6]
6. The method of claim 1, wherein the polynucleotide encoding the gamma -glutamylcysteine synthetase is a polynucleotide encoding a polypeptide consisting of the amino acid sequence set forth in SEQ ID NO: 1.
[claim7]
7. The method of claim 1, wherein the polynucleotide encoding the gamma -glutamylcysteine synthetase is a polynucleotide encoding a polypeptide which consists of an amino acid sequence with a deletion, a substitution, or an addition of not more than 10 amino acids in the amino acid sequence set forth in SEQ ID NO: 1 and which has a gamma -glutamylcysteine synthetase activity.
[claim8]
8. The method of claim 1, wherein the polynucleotide encoding the gamma -glutamylcysteine synthetase is a polynucleotide consisting of the nucleotide sequence set forth in SEQ ID NO: 2.
[claim9]
9. The method of claim 1, wherein the polynucleotide encoding the gamma -glutamylcysteine synthetase is a polynucleotide which hybridizes under a stringent condition defined by incubation overnight at 42 deg. C. in 5 * SSC and then washing in 0.1 * SSC at approximately 65 deg. C. with a polynucleotide consisting of a nucleotide sequence fully complementary to any one of (a) a polynucleotide consisting of the amino acid sequence set forth in SEQ ID NO: 1, (b) a polynucleotide encoding a polypeptide which consists of an amino acid sequence with deletion, substitution, or addition of one or several amino acids in the amino acid sequence set forth in SEQ ID NO: 1 and which has a gamma -glutamylcysteine synthetase activity, or (c) a polynucleotide consisting of the nucleotide sequence set forth in SEQ ID NO: 2.
[claim10]
10. The method of claim 1, wherein the alga is a sta6-positive strain of alga.
[claim11]
11. The method of claim 1, wherein the alga is a sta6-deficient strain of alga.
[claim12]
12. An alga produced by the method according to claim 1.
  • Inventor, and Inventor/Applicant
  • OGAWA KENICHI
  • NISHIKAWA MASANOBU
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
IPC(International Patent Classification)
Reference ( R and D project ) CREST Creation of Innovative Technologies to Control Carbon Dioxide Emissions AREA
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