TOP > 外国特許検索 > Protein crystallizing agent and method of crystallizing protein therewith

Protein crystallizing agent and method of crystallizing protein therewith コモンズ 新技術説明会

外国特許コード F130007438
整理番号 KG0049-US02
掲載日 2013年7月3日
出願国 アメリカ合衆国
出願番号 44965707
公報番号 20100095749
公報番号 8367412
出願日 平成19年9月19日(2007.9.19)
公報発行日 平成22年4月22日(2010.4.22)
公報発行日 平成25年2月5日(2013.2.5)
国際出願番号 JP2007068192
国際公開番号 WO2008102469
国際出願日 平成19年9月19日(2007.9.19)
国際公開日 平成20年8月28日(2008.8.28)
優先権データ
  • 特願2007-044275 (2007.2.23) JP
  • 2007WO-JP68192 (2007.9.19) WO
発明の名称 (英語) Protein crystallizing agent and method of crystallizing protein therewith コモンズ 新技術説明会
発明の概要(英語) (US8367412)
The present invention provides a technique for crystallizing a desired protein at a high probability; namely, a protein crystallizing agent and a method of crystallizing protein.
The present invention also provides a technique for determining the conditions for protein crystallization easily with high efficiency; namely, a method of screening the conditions for protein crystallization and a protein crystallization screening reagent.
As the protein crystallizing agent, at least one compound selected from the group consisting of a basic amino acid, acidic amino acid, ester derivative of amino acid and amide derivative of amino acid is used, or at least one of these compounds is used in combination with another protein crystallizing agent.
特許請求の範囲(英語) [claim1]
1. A method for crystallizing a protein, comprising the steps of: bringing a protein crystallizing agent into contact with a protein-containing solution, to prepare a mixture; and
precipitating and crystallizing the protein,
wherein the protein crystallizing agent comprises at least one compound selected from the group consisting of ornithine, an amide derivative of an amino acid, a salt thereof and a solvate thereof; and
wherein if the mixture contains ornithine or a salt thereof or a solvate thereof, then the ornithine or the salt thereof or the solvate thereof is contained in the mixture at a concentration of 20 mM or more.
[claim2]
2. The method for crystallizing a protein according to claim 1, wherein the protein is precipitated and crystallized in a vapor diffusion method, a batch method, a liquid-liquid diffusion method or a dialysis method.
[claim3]
3. The method for crystallizing a protein according to claim 1, wherein the amide derivative of an amino acid is glycinamide, serinamide, threonine amide, argininamide, or prolinamide.
[claim4]
4. A method for screening for protein crystallization conditions, comprising the steps of: bringing a protein crystallizing agent into contact with a protein-containing solution, to prepare a mixture; and
observing the occurrence of precipitation of the protein to screen for protein crystallization conditions,
wherein the protein crystallizing agent comprises at least one compound selected from the group consisting of ornithine, an amide derivative of an amino acid, a salt thereof and a solvate thereof; and
wherein if the mixture contains ornithine or a salt thereof or a solvate thereof, then the ornithine or the salt thereof or the solvate thereof is contained in the mixture at a concentration of 20 mM or more.
[claim5]
5. The method for screening for protein crystallization conditions according to claim 4, wherein the protein is precipitated and crystallized in a vapor diffusion method.
[claim6]
6. The method for screening for protein crystallization conditions according to claim 4, wherein the amide derivative of an amino acid is glycinamide, serinamide, threonine amide, argininamide, or prolinamide.
[claim7]
7. A method for crystallizing a protein, comprising the steps of: bringing a protein crystallizing agent into contact with a protein-containing solution, to prepare a mixture; and
precipitating and crystallizing the protein,
wherein the protein crystallizing agent comprises at least one compound selected from the group consisting of an amide derivative of an amino acid, a salt thereof and a solvate thereof.
[claim8]
8. The method for crystallizing a protein according to claim 7, wherein the amide derivative of an amino acid is glycinamide, serinamide, threonine amide, argininamide, or prolinamide.
[claim9]
9. A method for screening for protein crystallization conditions, comprising the steps of: bringing a protein crystallizing agent into contact with a protein-containing to prepare a mixture; and
observing the occurrence of precipitation of the protein to screen for protein crystallization conditions,
wherein the protein crystallizing agent comprises at least one compound selected from the group consisting of an amide derivative of an amino acid, a salt thereof and a solvate thereof.
[claim10]
10. The method for screening for protein crystallization conditions according to claim 9, wherein the amide derivative of an amino acid is glycinamide, serinamide, threonine amide, argininamide, or prolinamide.
  • 発明者/出願人(英語)
  • YAMAGUCHI HIROSHI
  • ITO LEN
  • KWANSEI GAKUIN
国際特許分類(IPC)
ライセンスをご希望の方、特許の内容に興味を持たれた方は、下記「問合せ先」までお問い合わせください。

PAGE TOP

close
close
close
close
close
close