Top > Search of International Patents > Protein crystallizing agent and method of crystallizing protein therewith

Protein crystallizing agent and method of crystallizing protein therewith commons meetings

Foreign code F130007438
File No. KG0049-US02
Posted date Jul 3, 2013
Country United States of America
Application number 44965707
Gazette No. 20100095749
Gazette No. 8367412
Date of filing Sep 19, 2007
Gazette Date Apr 22, 2010
Gazette Date Feb 5, 2013
International application number JP2007068192
International publication number WO2008102469
Date of international filing Sep 19, 2007
Date of international publication Aug 28, 2008
Priority data
  • P2007-044275 (Feb 23, 2007) JP
  • 2007WO-JP68192 (Sep 19, 2007) WO
Title Protein crystallizing agent and method of crystallizing protein therewith commons meetings
Abstract (US8367412)
The present invention provides a technique for crystallizing a desired protein at a high probability; namely, a protein crystallizing agent and a method of crystallizing protein.
The present invention also provides a technique for determining the conditions for protein crystallization easily with high efficiency; namely, a method of screening the conditions for protein crystallization and a protein crystallization screening reagent.
As the protein crystallizing agent, at least one compound selected from the group consisting of a basic amino acid, acidic amino acid, ester derivative of amino acid and amide derivative of amino acid is used, or at least one of these compounds is used in combination with another protein crystallizing agent.
Scope of claims [claim1]
1. A method for crystallizing a protein, comprising the steps of: bringing a protein crystallizing agent into contact with a protein-containing solution, to prepare a mixture; and
precipitating and crystallizing the protein,
wherein the protein crystallizing agent comprises at least one compound selected from the group consisting of ornithine, an amide derivative of an amino acid, a salt thereof and a solvate thereof; and
wherein if the mixture contains ornithine or a salt thereof or a solvate thereof, then the ornithine or the salt thereof or the solvate thereof is contained in the mixture at a concentration of 20 mM or more.
[claim2]
2. The method for crystallizing a protein according to claim 1, wherein the protein is precipitated and crystallized in a vapor diffusion method, a batch method, a liquid-liquid diffusion method or a dialysis method.
[claim3]
3. The method for crystallizing a protein according to claim 1, wherein the amide derivative of an amino acid is glycinamide, serinamide, threonine amide, argininamide, or prolinamide.
[claim4]
4. A method for screening for protein crystallization conditions, comprising the steps of: bringing a protein crystallizing agent into contact with a protein-containing solution, to prepare a mixture; and
observing the occurrence of precipitation of the protein to screen for protein crystallization conditions,
wherein the protein crystallizing agent comprises at least one compound selected from the group consisting of ornithine, an amide derivative of an amino acid, a salt thereof and a solvate thereof; and
wherein if the mixture contains ornithine or a salt thereof or a solvate thereof, then the ornithine or the salt thereof or the solvate thereof is contained in the mixture at a concentration of 20 mM or more.
[claim5]
5. The method for screening for protein crystallization conditions according to claim 4, wherein the protein is precipitated and crystallized in a vapor diffusion method.
[claim6]
6. The method for screening for protein crystallization conditions according to claim 4, wherein the amide derivative of an amino acid is glycinamide, serinamide, threonine amide, argininamide, or prolinamide.
[claim7]
7. A method for crystallizing a protein, comprising the steps of: bringing a protein crystallizing agent into contact with a protein-containing solution, to prepare a mixture; and
precipitating and crystallizing the protein,
wherein the protein crystallizing agent comprises at least one compound selected from the group consisting of an amide derivative of an amino acid, a salt thereof and a solvate thereof.
[claim8]
8. The method for crystallizing a protein according to claim 7, wherein the amide derivative of an amino acid is glycinamide, serinamide, threonine amide, argininamide, or prolinamide.
[claim9]
9. A method for screening for protein crystallization conditions, comprising the steps of: bringing a protein crystallizing agent into contact with a protein-containing to prepare a mixture; and
observing the occurrence of precipitation of the protein to screen for protein crystallization conditions,
wherein the protein crystallizing agent comprises at least one compound selected from the group consisting of an amide derivative of an amino acid, a salt thereof and a solvate thereof.
[claim10]
10. The method for screening for protein crystallization conditions according to claim 9, wherein the amide derivative of an amino acid is glycinamide, serinamide, threonine amide, argininamide, or prolinamide.
  • Inventor, and Inventor/Applicant
  • YAMAGUCHI HIROSHI
  • ITO LEN
  • KWANSEI GAKUIN
IPC(International Patent Classification)
Please contact us by E-mail or facsimile if you have any interests on this patent.

PAGE TOP

close
close
close
close
close
close