Top > Search of International Patents > Method for analyzing DNA methylation

Method for analyzing DNA methylation

Foreign code F130007493
Posted date Jul 10, 2013
Country United States of America
Application number 201113641923
Gazette No. 20130045478
Gazette No. 8980560
Date of filing Apr 21, 2011
Gazette Date Feb 21, 2013
Gazette Date Mar 17, 2015
International application number JP2011060339
International publication number WO2011132798
Date of international filing Apr 21, 2011
Date of international publication Oct 27, 2011
Priority data
  • P2010-098163 (Apr 21, 2010) JP
  • 2011WO-JP60339 (Apr 21, 2011) WO
Title Method for analyzing DNA methylation
Abstract (US8980560)
This invention provides a method for efficiently detecting DNA methylation.
The method for detecting DNA methylation comprises subjecting DNA to bisulfite treatment, subjecting DNA after bisulfite treatment to a first PCR, subjecting the resultant to nested PCR, and subjecting amplified DNA to denaturing gradient gel electrophoresis.
Scope of claims [claim1]
1. A method for detecting DNA methylation comprising steps of: subjecting DNA to bisulfite treatment;
subjecting the DNA after bisulfite treatment to a first PCR using the first set of primers corresponding to outer regions of the target region, the first set of primers selected from the group consisting of SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 30, and SEQ ID NO: 31;
subjecting the amplified DNA after the first PCR to a second PCR using the second set of primers corresponding to the target region, the second set of primers selected from the group consisting of SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO 10, SEQ ID NO: 11, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 32, and SEQ ID NO: 33; and
subjecting the amplified DNA after the second PCR to denaturing gradient gel electrophoresis,
wherein the annealing positions of the second set of primers are located inside the annealing positions of the first set of primers relative to the target region.
[claim2]
2. The method according to claim 1, wherein one primer from the second set of primers has a GC-clamp sequence at its 5' side.
[claim3]
3. The method according to claim 1, wherein the density gradient of the denaturing gradient gel is limited to a density gradient of a denaturing agent in the gel comprising acrylamide and the denaturing agent.
  • Inventor, and Inventor/Applicant
  • YOKOYAMA SEIYA
  • YONEZAWA SUGURU
  • KAGOSHIMA UNIVERSITY
IPC(International Patent Classification)
Please contact us by E-mail if you have any interests on this patent.

PAGE TOP

close
close
close
close
close
close