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IgA-binding peptide and purification of IgA using the same

Foreign code F130007494
Posted date Jul 10, 2013
Country United States of America
Application number 201113699579
Gazette No. 20130078741
Gazette No. 9593147
Date of filing May 24, 2011
Gazette Date Mar 28, 2013
Gazette Date Mar 14, 2017
International application number JP2011061906
International publication number WO2011148952
Date of international filing May 24, 2011
Date of international publication Dec 1, 2011
Priority data
  • P2010-118508 (May 24, 2010) JP
  • 2011WO-JP61906 (May 24, 2011) WO
Title IgA-binding peptide and purification of IgA using the same
Abstract (US9593147)
This invention relates to: a peptide which comprises an amino acid sequence consisting of 12 to 18 amino acid residues represented by (X1-3)―C―(X8-10)―C―(X1-3) wherein each X independently represents an arbitrary amino acid residue other than cysteine, and C represents a cysteine residue, and is capable of binding to human IgA; and to a method for analyzing or purifying human IgA using the peptide.
Scope of claims [claim1]
1. A peptide which consists of any of the following amino acid sequences 1) to 7): 1) QMRCLSYKGRRVCLWL (SEQ ID NO: 2),
2) KRLCLQYKGSKVCFRL (SEQ ID NO: 4),
3) RMRCLTYRGRRVCLEL (SEQ ID NO: 5),
4) SMRCLQYRGSRVCLTL (SEQ ID NO: 6),
5) HLRCLRYKGTRVCFSL (SEQ ID NO: 8),
6) HVRCLSYKGREVCVQL (SEQ ID NO: 9), and
7) PRMCLHYKGRRVCIPY (SEQ ID NO: 10),
wherein the peptide is capable of binding to human IgA, and
wherein said peptide is optionally labeled, linked to a protein as part of a fusion protein, or bound to a solid phase.
[claim2]
2. The peptide according to claim 1, wherein the peptide has a disulfide bond between its two cysteine (C) residues.
[claim3]
3. The peptide according to claim 1, wherein the peptide is capable of binding to serum (monomeric) IgA and secretory (dimeric) IgA.
[claim4]
4. The peptide according to claim 1, wherein the peptide is labeled.
[claim5]
5. A fusion protein comprising a protein linked to a peptide according to claim 1.
[claim6]
6. An immobilized peptide comprising a peptide according to claim 1 bound to a solid phase.
[claim7]
7. A nucleic acid encoding a peptide according to claim 1.
[claim8]
8. A method for purifying IgA comprising binding IgA to A) a peptide which consists of any of the following amino acid sequences 1) to 7): 1) QMRCLSYKGRRVCLWL (SEQ ID NO: 2),
2) KRLCLQYKGSKVCFRL (SEQ ID NO: 4),
3) RMRCLTYRGRRVCLEL (SEQ ID NO: 5),
4) SMRCLQYRGSRVCLTL (SEQ ID NO: 6),
5) HLRCLRYKGTRVCFSL (SEQ ID NO: 8),
6) HVRCLSYKGREVCVQL (SEQ ID NO: 9), and
7) PRMCLHYKGRRVCIPY (SEQ ID NO: 10),
wherein the peptide is capable of binding to human IgA, or
(B) an immobilized peptide according to claim 6; and
releasing the bound IgA to collect the purified IgA.
[claim9]
9. A method for detecting IgA comprising binding IgA in a sample to A) a peptide that consists of any of the following amino acid sequences 1) to 7): 1) QMRCLSYKGRRVCLWL (SEQ ID NO: 2),
2) KRLCLQYKGSKVCFRL (SEQ ID NO: 4),
3) RMRCLTYRGRRVCLEL (SEQ ID NO: 5),
4) SMRCLQYRGSRVCLTL (SEQ ID NO: 6),
5) HLRCLRYKGTRVCFSL (SEQ ID NO: 8),
6) HVRCLSYKGREVCVQL (SEQ ID NO: 9), and
7) PRMCLHYKGRRVCIPY (SEQ ID NO: 10),
wherein the peptide is capable of binding to human IgA, or
(B) an immobilized peptide according to claim 6; and
detecting bound IgA.
[claim10]
10. A kit for analysis or purification of human IgA comprising (A) at least one peptide that consists of any of the following amino acid sequences 1) to 7): 1) QMRCLSYKGRRVCLWL (SEQ ID NO: 2),
2) KRLCLQYKGSKVCFRL (SEQ ID NO: 4),
3) RMRCLTYRGRRVCLEL (SEQ ID NO: 5),
4) SMRCLQYRGSRVCLTL (SEQ ID NO: 6),
5) HLRCLRYKGTRVCFSL (HQ ID NO: 8),
6) HVRCLSYKGREVCVQL (HQ ID NO: 9), and
7) PRMCLHYKGRRVCIPY (HQ ID NO: 10),
wherein the peptide is capable of binding to human IgA, or
(B) an immobilized peptide according to claim 6.
[claim11]
11. A column for IgA separation, comprising an immobilized peptide according to claim 6.
[claim12]
12. A peptide consisting of any of the following amino acid sequences 1) to 7): 1) QMRCLSYKGRRVCLWL (SEQ ID NO: 2),
2) KRLCLQYKGSKVCFRL (SEQ ID NO: 4),
3) RMRCLTYRGRRVCLEL (SEQ ID NO: 5),
4) SMRCLQYRGSRVCLTL (SEQ ID NO: 6),
5) HLRCLRYKGTRVCFSL (SEQ ID NO: 8),
6) HVRCLSYKGREVCVQL (SEQ ID NO: 9), and
7) PRMCLHYKGRRVCIPY (SEQ ID NO: 10),
wherein the peptide is capable of binding to human IgA.
  • Inventor, and Inventor/Applicant
  • ITO YUJI
  • KAGOSHIMA UNIVERSITY
  • OTSUKA CHEMICAL
IPC(International Patent Classification)
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