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IgG-BINDING PEPTIDE AND METHOD FOR DETECTING AND PURIFYING IgG USING SAME

外国特許コード F130007528
掲載日 2013年7月16日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2012JP071303
国際公開番号 WO 2013027796
国際出願日 平成24年8月23日(2012.8.23)
国際公開日 平成25年2月28日(2013.2.28)
優先権データ
  • 特願2011-182539 (2011.8.24) JP
発明の名称 (英語) IgG-BINDING PEPTIDE AND METHOD FOR DETECTING AND PURIFYING IgG USING SAME
発明の概要(英語) Provided is a peptide capable of binding to human IgG specifically or selectively. A peptide characterized by comprising an amino acid sequence represented by formula (I): (X1-3)-C-(X2)-H-R-G-(Xaa1)-L-V-W-C-(X1-3) (wherein Xs independently represent an arbitrary amino acid residue other than a cysteine residue; C represents a cysteine residue; H represents a histidine residue; R represents an arginine residue; G represents a glycine residue; Xaa1 represents a glutamic acid residue or an asparagine residue; L represents a leucine residue; V represents a valine residue; and W represents a tryptophan residue) and composed of 13-17 amino acid residues, and also characterized by being capable of binding to human IgG.
従来技術、競合技術の概要(英語) BACKGROUND ART
Current, antibody pharmaceuticals is, most highly reliable attracted attention and is the molecular target as a medicament, and rapidly increased in the field of new drugs. Used currently in development or most of the immunoglobulin g antibody pharmaceuticals (hereinafter referred to as' IgG ') is to use the antibodies belonging to class.
Conventionally, for the purification of antibodies IgG, g Staphylococcus aureus protein A or protein derived from a protein is used (non-patent document 1, 2). These proteins, mouse, rabbit IgG for binding to, at the level of the research reagent has been widely used as the purified IgG, in recent years with respect to the human IgG1 antibody pharmaceuticals, and to be employed as the pharmaceutical field, industrial, pharmaceutical has been increasing importance in the use. In particular protein A column, in the purification of antibody pharmaceuticals also play a central role, the manufacturer of the many antibody pharmaceuticals, purification system around the column was introduced.
However, in order that a protein A column, some problems have been noted. 1 Is one, into the purified antibody is the problem of protein A. And protein A or a protein derived from bacteria, highly immunogenic after administration of the human body, the endotoxin contamination is concerned. As the affinity ligand is this medicaments such as used for purification, so as to avoid a contamination of unwanted substances, a high purity protein A as a ligand and it is required, this is utilized for the purification protein A pharmaceutical raises the cost of the column are a cause of the.
In order to solve such a problem, the development of new IgG antibody purification system is being performed. For example, protein A (non-patent document 3, 4) or mimetic peptides, and protein A IgG Fc antibody and X-ray crystal structure of the designed group, non-peptidic affinity ligands has been reported (non-patent document 5) however, they are a problem in a binding capacity and specificity is a limitation in use.
In addition, using synthetic peptide phage library or the like, to search for new IgG-binding peptides as research has been made (Patent Document 1-3).
In this way, as new peptides, low-molecular-weight by antibody purification IgG but research has been conducted, protein A, g column will vary, the industrial scale can be handled by the new purification system is not present, still in the art, a new approach for purifying the antibody IgG is demanded.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • OTSUKA CHEMICAL CO., LTD.
  • KAGOSHIMA UNIVERSITY
  • 発明者(英語)
  • ITO Yuji
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IS JP KE KG KM KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PE PG PH PL PT QA RO RS RU RW SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG
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