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YEAST HAVING FREEZING STRESS RESISTANCE

外国特許コード F130007550
整理番号 S2012-0188-N0
掲載日 2013年7月16日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2012JP080058
国際公開番号 WO 2013088920
国際出願日 平成24年11月20日(2012.11.20)
国際公開日 平成25年6月20日(2013.6.20)
優先権データ
  • 特願2011-274519 (2011.12.15) JP
発明の名称 (英語) YEAST HAVING FREEZING STRESS RESISTANCE
発明の概要(英語) Provided are the following: a yeast the freezing stress resistance of which is increased and which is characterized in that the POG1 gene is inactivated; a method for producing such a yeast; and a method for using the yeast in the production of a food product.
従来技術、競合技術の概要(英語) BACKGROUND ART
Pan, of the product by fermentation of yeast in dough manufacturing expansion processes needs to be made, constant quality bread in order to provide, baker's yeast, Saccharomyces, cerevisiae (Saccharomyces cerevisiae) is required a careful control of the fermentation. Yeast cells, the bread in the course, freezing, drying, high temperature, and high osmolality of the many environments and subjected to a stress, (non-patent document 1), these environmental stresses include, results in a decrease of the yeast fermentation. For this reason, a yeast strain having a resistance to the environmental stress is the production of the desired to establish a technique and, in recent years, a pan made of frozen dough method is rapidly spreading from the fact that, in the method of the freezing tolerance to stress on the additional growth of the yeast strain, particularly desired.
And yeast manufacturing industry is the bread industry, bread yeast fermentation when loaded and there is an approach to limit environmental stress, many, these yeast resistance to environmental stress by the production of attempting to solve the problem.
On the other hand, with the accumulation of knowledge with respect to stress tolerance mechanisms, by altering the resistance-associated gene has been made an attempt to enhance resistance. For example, one of the 1 amino acid proline, freezing or drying, oxidation or the like from the stress of the yeast (Patent Document 1) have the property of protection, guru tamil kinase variants encoding γ - PRO1 gene, disruption of the gene encoding proline oxidase strain PUT1 proline is accumulated in the cells can be ethanol-resistant is known (patent document 2). In addition, acetyltransferase N- is Mpr1, yeast, heat shock, hydrogen peroxide, ethanol, and used to protect from oxidative stress and low temperature such as (non-patent document 2 from 4), variant Mpr1 dry yeast expression efficiently, have resistance to high temperatures (Patent Document 3) are known. In addition, by the absence of the CBS2 gene, can be susceptible to drought stress (non-patent document 5), by disruption of the gene DBF2, becomes highly sensitive sorbitol stress (non-patent document 6), aromatic amino acids (such as ARO1) of the gene involved in sucrose-type strain of considerable deterioration in stress resistance, BUD23, GON7, SPT20 of the gene disruption line is high stress sucrose (non-patent document 7) sensitized, phosphate substrate for dephosphorylation of the protein encoding a phosphatase of the OCA1 gene or OCA2 gene, or disruption of a gene for strains ALD2 sucrose increases the stress resistance (patent document 4) and the like are known. With respect to freezing stress tolerance, disruption of the gene encoding the acidic trehalase strain ATH1-sucrose frozen dough stress tolerance and enhanced stress resistance (patent document 5), a disruption of the gene encoding arginase CAR1 strain, a significant quantity of intracellular amino acids high polarity is stored, increases the freezing stress resistance (patent document 6), strain SNF5 PMR1 gene or a disruption of the gene is impaired freezing stress tolerance (non-patent document 8) and the like are known.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • NATIONAL UNIVERSITY CORPORATION NARA INSTITUTE OF SCIENCE AND TECHNOLOGY
  • 発明者(英語)
  • TAKAGI, Hiroshi
  • SASANO, Yu
  • SHIMA, Jun
  • HAITANI, Yutaka
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IS JP KE KG KM KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG
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