DESIGN METHOD FOR RNA-BINDING PROTEIN USING PPR MOTIF, AND USE THEREOF
Provided is a method for designing a protein capable of selectively binding RNA bases or specifically binding an RNA base sequence. This protein includes 1 or more (preferably 2-14) PPR motifs comprising a polypeptide represented by formula (1) and having a length of 30-38 amino acids (in formula (1): Helix A is a moiety which is represented by formula (2), has a length of 12 amino acids, and is capable of forming an alpha-helix structure (in formula (2), A1-A12 each independently represent an amino acid)
X is a moiety which is either not present or which has a length of 1-9 amino acids
Helix B is a moiety that has a length of 11-13 amino acids, and is capable of forming an alpha-helix structure
L is a moiety which is represented by formula (3) and has a length of 2-7 amino acids (in formula (3), each amino acid is numbered from the C-terminus in the following manner "i"(-1), "ii"(-2), on condition that in some cases Liii to Lvii are not present)). The protein corresponding to the RNA bases or base sequence that will be bound is obtained by combining: A1, A4, and 3 Lii amino acids
or A4 and 2 Lii amino acids.