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Vesicles with encapsulated substance and production method therefor

Foreign code F130007600
File No. AF12-07WO
Posted date Aug 9, 2013
Country China
Application number 201180034554
Gazette No. 103002982
Gazette No. 103002982
Date of filing May 23, 2011
Gazette Date Mar 27, 2013
Gazette Date Jun 8, 2016
International application number JP2011061790
International publication number WO2011145745
Date of international filing May 23, 2011
Date of international publication Nov 24, 2011
Priority data
  • 2011JP061790 (May 23, 2011) WO
  • P2010-117821 (May 21, 2010) JP
  • P2010-117823 (May 21, 2010) JP
Title Vesicles with encapsulated substance and production method therefor
Abstract Provided is a method for easily and efficiently producing encapsulated substance vesicles wherein a substance is encapsulated in the cavity of vesicles obtained by polymer self-assembly.
Empty vesicles that have membranes comprising a first polymer that is a block copolymer with uncharged hydrophilic segments and a first kind of charged segments and a second polymer with a second kind of charged segments that carry a charge that is the opposite of said first kind of charged segments as well as spaces that are enclosed by said membranes are mixed in an aqueous medium with the substance that is to be encapsulated in the spaces.
Scope of claims [claim1]
1. the method for packing bubble in the material that is surrounded by material in being manufactured in the space part, contain following operation:
Prepare the operation of empty pocket bubble, wherein, described empty pocket bubble has film and has by described membrane-enclosed space part, described film contains the first polymer and the second polymer, described the first polymer is the block copolymer with non-charged hydrophilic segment and first charged segment, and described the second polymer has the second charged segment that carries with the described first charged segment opposite charges; And
With the operation that described empty pocket steeps and described material mixes in aqueous medium.
[claim2]
2. method according to claim 1, wherein, described mixing by be selected from by stir, the gimmick of group that vibration, collision consist of carries out.
[claim3]
3. method according to claim 1 and 2, wherein, the described film of described empty pocket bubble has the three-decker that is made of outer, intermediate layer and internal layer.
[claim4]
4. each described method according to claim 1~3, wherein, described material is selected from the group that is made of biomolecule, organic compound and inorganic substances.
[claim5]
5. each described method according to claim 1~4, wherein, the first polymer is the block copolymer that contains non-charge hydrophilic segment and anionic segments, the second polymer is the block copolymer that contains non-charge hydrophilic segment and cation chain segments, and/or contains cation chain segments but do not contain the polymer of non-charge hydrophilic segment.
[claim6]
6. each described method according to claim 1~4, wherein, the first polymer is the block copolymer that contains non-charge hydrophilic segment and cation chain segments, the second polymer is the block copolymer that contains non-charge hydrophilic segment and anionic segments, and/or contains anionic segments but do not contain the polymer of non-charge hydrophilic segment.
[claim7]
7. according to claim 5 or 6 described methods, wherein, described anionic segments is nucleic acid.
[claim8]
8. each described method according to claim 5~7, wherein, described cation chain segments is polyamine.
[claim9]
9. each described method according to claim 5~8, wherein, described non-charged hydrophilic segment is PAG.
[claim10]
10. vesica, contain film, by space part that described film surrounded, and described space part in the material that wraps in the institute, wherein said film comprises the first polymer and the second polymer, described the first polymer is the block copolymer with non-charged hydrophilic segment and first charged segment, and described the second polymer has the second charged segment that carries with the described first charged segment opposite charges.
[claim11]
11. vesica according to claim 10, wherein, described film has the three-decker that is made of skin, intermediate layer and internal layer.
[claim12]
12. according to claim 10 or 11 described vesicas, wherein, described material is selected from the group that is made of biomolecule, organic compound and inorganic substances.
[claim13]
13. each described vesica according to claim 10~12, wherein, the first polymer is the block copolymer that contains non-charge hydrophilic segment and anionic segments, the second polymer is the block copolymer that contains non-charge hydrophilic segment and cation chain segments, and/or contains cation chain segments but do not contain the polymer of non-charge hydrophilic segment.
[claim14]
14. each described vesica according to claim 10~13, wherein, the first polymer is the block copolymer that contains non-charge hydrophilic segment and cation chain segments, the second polymer is the block copolymer that contains non-charge hydrophilic segment and anionic segments, and/or contains anionic segments but do not contain the polymer of non-charge hydrophilic segment.
[claim15]
15. according to claim 13 or 14 described vesicas, wherein, described anionic segments is nucleic acid.
[claim16]
16. each described vesica according to claim 13~15, wherein, described cation chain segments is polyamine.
[claim17]
17. each described vesica according to claim 13~16, wherein, described non-charged hydrophilic segment is PAG.
[claim18]
18. a vesica has film and has by described membrane-enclosed space part, wherein said film contains block copolymer with non-charged hydrophilic segment and cation chain segments, and nucleic acid.
[claim19]
19. vesica according to claim 18, wherein, described film has the three-decker that is made of skin, intermediate layer and internal layer.
[claim20]
20. according to claim 18 or 19 described vesicas, wherein, the cation radical of described cation chain segments is N to the mol ratio of the phosphate of described nucleic acid +/ P ratio is above 1.0 and be lower than 3.0.
[claim21]
21. each described vesica according to claim 18~20, wherein, described non-charged hydrophilic segment is PAG.
[claim22]
22. each described vesica according to claim 18~21, wherein, described cation chain segments is polyamine.
[claim23]
23. each described vesica according to claim 18~22, wherein, described film also contains crosslinking agent.
[claim24]
24. vesica according to claim 23, wherein, described crosslinking agent is that the CL/N ratio is more than 0.1 to the mol ratio of the cation radical of described cation chain segments.
[claim25]
25. according to claim 23 or 24 described vesicas, wherein, described crosslinking agent is glutaraldehyde.
[claim26]
26. each described vesica according to claim 10~25 is as drug delivery system.
  • Applicant
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • Inventor
  • KATAOKA KAZUNORI
  • KISHIMURA AKIHIRO
  • ANRAKU YASUTAKA
  • MIYATA KANJIRO
  • CHUANOI SAYAN
  • SUMA TOMOYA
  • OBA MAKOTO
IPC(International Patent Classification)
Reference ( R and D project ) CREST Establishment of Innovative Manufacturing Technology Based on Nanoscience AREA
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