METHOD FOR PREPARING AMNIOTIC MESENCHYMAL STEM CELL, AND ISOLATED AMNIOTIC MESENCHYMAL STEM CELL MASS

• 特願2011-257419 (2011.11.25) JP

An amniotic mesenchymal stem cell mass having a high proliferation ability and a high differentiation ability can be produced by a method comprising the steps of: (A) collecting a cell mass of a mesenchymal cell from the amnion of a mammal

(B) subjecting the cell mass of the mesenchymal cell to flow cytometry to thereby sort cells, wherein a specific gate is provided in a scattergram produced in the flow cytometry

(C) and subjecting the sorted cells to subculture. Alternatively, the amniotic mesenchymal stem cell mass having a high proliferation ability and a high differentiation ability can also be produced by a method comprising the steps of: (D) collecting a cell mass of a mesenchymal cell from the amnion of a mammal

(E) subjecting the collected cell mass to initial culture for 2 to 3 days

(F) repeating the subculture of the resultant cell mass three to four times at a low cell concentration

and (G) maintaining the culture of the cells in the same culture dish until the cells become confluent when a fusiform colony of the cells is formed in the subculture.

• UNIVERSITY OF TOYAMA

• NIKAIDO, TOSHIO,
• YOSHIDA, TOSHIKO,
• OKABE, MOTONORI,
• KOIKE, CHIKA,
• NOGAMI, MAKIKO,
• KIMURA, TOMOATSU,
• NOGUCHI, MAKOTO,
• TSUNO, HIROAKI,
• TAKEDA, YUJI

• C12N   5/0775    間葉系幹細胞；脂肪組織由来幹細胞
• C12N   5/077     間葉細胞，例．骨細胞，軟骨細胞，骨髄間質細胞，脂肪細胞または筋肉細胞