METHOD FOR PREPARING AMNIOTIC MESENCHYMAL STEM CELL, AND ISOLATED AMNIOTIC MESENCHYMAL STEM CELL MASS
外国特許コード | F130007708 |
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掲載日 | 2013年11月27日 |
出願国 | 世界知的所有権機関(WIPO) |
国際出願番号 | 2012JP080375 |
国際公開番号 | WO 2013/077428 |
国際出願日 | 平成24年11月22日(2012.11.22) |
国際公開日 | 平成25年5月30日(2013.5.30) |
優先権データ |
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発明の名称 (英語) | METHOD FOR PREPARING AMNIOTIC MESENCHYMAL STEM CELL, AND ISOLATED AMNIOTIC MESENCHYMAL STEM CELL MASS |
発明の概要(英語) |
An amniotic mesenchymal stem cell mass having a high proliferation ability and a high differentiation ability can be produced by a method comprising the steps of: (A) collecting a cell mass of a mesenchymal cell from the amnion of a mammal (B) subjecting the cell mass of the mesenchymal cell to flow cytometry to thereby sort cells, wherein a specific gate is provided in a scattergram produced in the flow cytometry (C) and subjecting the sorted cells to subculture. Alternatively, the amniotic mesenchymal stem cell mass having a high proliferation ability and a high differentiation ability can also be produced by a method comprising the steps of: (D) collecting a cell mass of a mesenchymal cell from the amnion of a mammal (E) subjecting the collected cell mass to initial culture for 2 to 3 days (F) repeating the subculture of the resultant cell mass three to four times at a low cell concentration and (G) maintaining the culture of the cells in the same culture dish until the cells become confluent when a fusiform colony of the cells is formed in the subculture. |
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国際特許分類(IPC) |
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日本語項目の表示
発明の名称 | 羊膜間葉系幹細胞の調製方法および単離された羊膜間葉系幹細胞集団 |
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『 METHOD FOR PREPARING AMNIOTIC MESENCHYMAL STEM CELL, AND ISOLATED AMNIOTIC MESENCHYMAL STEM CELL MASS 』に関するお問合せ
- 国立大学法人 富山大学 研究推進機構 産学連携推進センター 知財・リエゾンオフィス
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