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METHOD FOR PREPARING AMNIOTIC MESENCHYMAL STEM CELL, AND ISOLATED AMNIOTIC MESENCHYMAL STEM CELL MASS

Foreign code F130007708
Posted date Nov 27, 2013
Country WIPO
International application number 2012JP080375
International publication number WO 2013/077428
Date of international filing Nov 22, 2012
Date of international publication May 30, 2013
Priority data
  • P2011-257419 (Nov 25, 2011) JP
Title METHOD FOR PREPARING AMNIOTIC MESENCHYMAL STEM CELL, AND ISOLATED AMNIOTIC MESENCHYMAL STEM CELL MASS
Abstract

An amniotic mesenchymal stem cell mass having a high proliferation ability and a high differentiation ability can be produced by a method comprising the steps of: (A) collecting a cell mass of a mesenchymal cell from the amnion of a mammal

(B) subjecting the cell mass of the mesenchymal cell to flow cytometry to thereby sort cells, wherein a specific gate is provided in a scattergram produced in the flow cytometry

(C) and subjecting the sorted cells to subculture. Alternatively, the amniotic mesenchymal stem cell mass having a high proliferation ability and a high differentiation ability can also be produced by a method comprising the steps of: (D) collecting a cell mass of a mesenchymal cell from the amnion of a mammal

(E) subjecting the collected cell mass to initial culture for 2 to 3 days

(F) repeating the subculture of the resultant cell mass three to four times at a low cell concentration

and (G) maintaining the culture of the cells in the same culture dish until the cells become confluent when a fusiform colony of the cells is formed in the subculture.

  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • UNIVERSITY OF TOYAMA
  • Inventor
  • NIKAIDO, TOSHIO,
  • YOSHIDA, TOSHIKO,
  • OKABE, MOTONORI,
  • KOIKE, CHIKA,
  • NOGAMI, MAKIKO,
  • KIMURA, TOMOATSU,
  • NOGUCHI, MAKOTO,
  • TSUNO, HIROAKI,
  • TAKEDA, YUJI
IPC(International Patent Classification)
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