TOP > 外国特許検索 > METHOD FOR PRODUCING PANCREATIC HORMONE-PRODUCING CELL, PANCREATIC HORMONE-PRODUCING CELL, AND DIFFERENTIATION/INDUCTION PROMOTER

METHOD FOR PRODUCING PANCREATIC HORMONE-PRODUCING CELL, PANCREATIC HORMONE-PRODUCING CELL, AND DIFFERENTIATION/INDUCTION PROMOTER

外国特許コード F130007791
整理番号 S2012-0753-N0
掲載日 2013年12月18日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2013JP064469
国際公開番号 WO 2013176249
国際出願日 平成25年5月24日(2013.5.24)
国際公開日 平成25年11月28日(2013.11.28)
優先権データ
  • 特願2012-120281 (2012.5.25) JP
発明の名称 (英語) METHOD FOR PRODUCING PANCREATIC HORMONE-PRODUCING CELL, PANCREATIC HORMONE-PRODUCING CELL, AND DIFFERENTIATION/INDUCTION PROMOTER
発明の概要(英語) Provided are: a method for producing (a method for differentiating/inducing) a pancreatic hormone-producing cell, which enables the highly efficient differentiation/induction of a pluripotent stem cell or a pancreatic tissue stem/progenitor cell into a pancreatic hormone-producing cell; a pancreatic hormone-producing cell produced by the method; and a differentiation/induction promoter for use in the production method. The method for producing a pancreatic hormone-producing cell according to the present invention is characterized in that a specific differentiation/induction promoter is added to a culture medium in the process of differentiating/inducing of a pluripotent stem cell or a pancreatic tissue stem/progenitor cell into the pancreatic hormone-producing cell. The differentiation/induction promoter to be used is: a polypeptide which comprises an amino acid sequence encoded by DNA comprising the nucleotide sequence represented by SEQ ID NO: 1 or a variant thereof; or a culture supernatant of a cell into which DNA comprising the nucleotide sequence represented by SEQ ID NO: 1 or DNA capable of hybridizing with DNA comprising a nucleotide sequence complementary to the aforementioned DNA under stringent conditions is integrated as a foreign gene.
従来技術、競合技術の概要(英語) BACKGROUND ART
The pancreas exocrine and endocrine cells, endocrine and exocrine play important roles in both of the responsible for the organ. Endocrine cells producing pancreatic hormones, play a role in secreting, glucagon from α cells, β cells from insulin, somatostatin is δ cells, pancreatic polypeptide are respectively PP from cells known to be secreted. In particular insulin is a reduction in blood glucose level, blood sugar level maintained at the correct play an important role.
In recent years, pluripotent stem cells or pancreatic tissue stem/progenitor cells differentiated into pancreatic hormones producing cells from the method for inducing a large number have been reported (non-patent document 1-4, refer to Patent Document 1-6). Such efficiently removed by a method to induce the differentiation of pancreatic hormone-producing cells can be obtained, as a substitute for transplanting pancreatic islet according to the method of vibration and expected. Further, or pluripotent stem cell derived from a patient in the pancreatic tissue stem/progenitor cells from the pancreatic hormones producing cells it is possible to obtain, without the problem of rejection is contemplated.
However, this method has been reported to induce the differentiation of, both pancreatic hormones producing cells differentiation efficiency was not sufficient. For example, in non-patent document 1 efficiency of differentiation into insulin-producing cells is about 12%. For this reason, pluripotent stem cells or pancreatic tissue stem/progenitor cells from the pancreatic hormones producing cells to a high efficiency method for inducing differentiation capable of inducing differentiation has been desired. In addition, in non-patent document 3, mouse pdx1 gene is introduced into the ES cells into insulin-producing cells by culturing are induced to differentiate with to, from the viewpoint of safety, a method for inducing differentiation without gene transfer is preferred.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • SAITAMA MEDICAL UNIVERSITY
  • 発明者(英語)
  • TOYOSHIMA, Hideo
  • OKAZAKI, Yasushi
  • YOKOO, Tomotaka
  • SUGAWARA, Izumi
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IS JP KE KG KM KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

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