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GENE FOR SHORTENING STALK OF GRAMINEOUS PLANT AND METHOD FOR PRODUCING SHORT-STALKED GRAMINEOUS PLANT

Foreign code F140007839
File No. S2013-1211-N0
Posted date Feb 18, 2014
Country WIPO
International application number 2013JP056339
International publication number WO 2013146171
Date of international filing Mar 7, 2013
Date of international publication Oct 3, 2013
Priority data
  • P2012-077453 (Mar 29, 2012) JP
Title GENE FOR SHORTENING STALK OF GRAMINEOUS PLANT AND METHOD FOR PRODUCING SHORT-STALKED GRAMINEOUS PLANT
Abstract The purpose of the invention is to identify a short-stalk gene other than the sd1 gene and produce a short-stalked gramineous plant by utilizing a short-stalk gene other than the sd1 gene. The present invention provides a method for producing a short-stalked gramineous plant utilizing gene d60 for shortening the stalk of a gramineous plant, wherein the method for producing a short-stalked gramineous plant is characterized in that the expression of Os02g0280200 is suppressed. The present invention also provides a short-stalked gramineous plant and the like in which the expression of Os02g0280200 is suppressed.
Outline of related art and contending technology BACKGROUND ART
Is grass, rice, wheat, barley, oats, rye, millet, millet, Setaria, corn, finger millet, sorghum and the like agriculturally important plant. Is a long grass culm, strong wind, for example, by the falling-down and it is not a typhoon, harvesting problem is greatly reduced.
Therefore, it is difficult to develop a kind of product is falling, gene obtained short culm, many years of breeding of a grass pollen short culm over are carried out. However, the short stature of the plant body is also reduced by making the unhusked ear or culm in many cases, gene is such a short-culm, not desirable from the viewpoint of productivity. Therefore, the unhusked ear and does not affect the, preparing the short stature gene only is required to shorten the culm.
Thus far found, and, in practical use only sd1 gene is a short culm. Is sd1, referred to as semi-dwarf, but the length of the ear is completely exposed whole plant of low stature trait gene, gibberellin (GA) enzyme in the biosynthesis systems of the type C20 gene defect. Current, Calrose 76 of the US, IR36 of the south-east Asia, such as Japan Milky NEW, short stone goods in the world rice at the cultivated species, as these short stone goods as a result of a genetic analysis for a given species, all of the type of semi-dwarf gene in sd1 was found to have the same locus. That is, a grass pollen of the current can be cultivated by short culm, slightly 1 dominated by one type of a particular gene, one type of a particular gene in a wide range slightly 1 used there is the drawback that occurs. Maintain the genetic diversity of the species, for the purposes of breeding to extend the functionalities, without resorting to genes were only sd1, the falling-down resistance gene of another short-breeding utilization in a culm propel and should, gene has been desired in the new short culm.
In recent years, the newly discovered is semi-dwarf gene d60 (non-patent document 1 and 2). Is d60, and a plant that does not have the gene as compared to only about 20 cm short stature gene which in the culm.
Semi-dwarf gene is d60, ubiquitous lodging coexist with gal female gametes of the lethal gene and the dose lethal for gametes, Koshihikari d60 system, d60 hokuriku having No. 100 type, and other varieties (genotype d60d60GalGal) system, for crossing (D60D60galgal) system F1 (D60d60Galgal) in, pollen and seed fertility is 75%, progeny F2 6 fertile in length (4D60D60: 2D60d60GalGal) culm: 2 (D60d60Galgal=F1 type) sterile portion length culm: ratio of the short 1 (d60d60GalGal) specifically separating the culm genetic manner take. Therefore, gal genetic d60 is essential. d60 Is an artifact of the gal without simultaneous mutation could not be obtained in a naturally occurring gene valuable short culm.
Semi-dwarf gene is d60, 2 of a grass pollen of the first found to be present on the chromosome, the first 1 to sd1 present on the chromosome is a genetically and functionally independent gene was shown to be (patent document 1). Further, using DNA marker or genetic marker, the presence of the gene on the chromosome 2 d60 of the first and/or d60 gene on a chromosome that is essential for the first genetic gal 5 by determining the presence of the gene, d60 gene and selecting a plant belonging to the family Gramineae gal gene has been developed a method (Patent Document 1).
However, to date, no gene has been identified and d60, the short culmed function has not been elucidated, has not yet been put to a practical use.
Scope of claims (In Japanese)請求の範囲 [請求項1]
 イネ科植物において、Os02g0280200遺伝子の発現を抑制することを特徴とする、短稈イネ科植物の作出方法。

[請求項2]
 アンチセンス法または突然変異誘発法によってOs02g0280200遺伝子の発現を抑制することを特徴とする、請求項1記載の方法。

[請求項3]
 Os02g0280200遺伝子の全長cDNAのアンチセンス配列を含有するベクターでイネ科植物を形質転換することを特徴とする、請求項2記載の方法。

[請求項4]
 Os02g0280200遺伝子の第2エキソンのアンチセンス配列を含有するベクターでイネ科植物を形質転換することを特徴とする、請求項2記載の方法。

[請求項5]
 Os02g0280200遺伝子の塩基配列の479位にチミンからシトシンへのヌクレオチド変異を導入することを特徴とする、請求項2記載の方法。

[請求項6]
 配列番号2で示される塩基配列を含有するベクターでイネ科植物を形質転換することを特徴とする、請求項5記載の方法。

[請求項7]
 配列番号4で示されるアミノ酸配列を含むタンパク質をコードする塩基配列を含有するベクターでイネ科植物を形質転換することを特徴とする、請求項5記載の方法。

[請求項8]
 配列番号6で示される塩基配列を含有するベクターでイネ科植物を形質転換することを特徴とする、請求項5記載の方法。

[請求項9]
 配列番号8で示されるアミノ酸配列を含むタンパク質をコードする塩基配列を含有するベクターでイネ科植物を形質転換することを特徴とする、請求項5記載の方法。

[請求項10]
 Os02g0280200遺伝子の発現が抑制された、短稈イネ科植物。

[請求項11]
 配列番号2で示される塩基配列を含む短稈遺伝子。

[請求項12]
 配列番号4で示されるアミノ酸配列を含むタンパク質をコードする短稈遺伝子。

[請求項13]
 Os02g0280200遺伝子の全長cDNAのアンチセンス配列からなるDNA。

[請求項14]
 Os02g0280200遺伝子の第2エキソンのアンチセンス配列からなるDNA。

[請求項15]
 配列番号3で示されるアミノ酸配列を含むタンパク質をコードする塩基配列のアンチセンス配列からなるDNA。

[請求項16]
 配列番号7で示されるアミノ酸配列を含むタンパク質をコードする塩基配列のアンチセンス配列からなるDNA。

  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • NATIONAL UNIVERSITY CORPORATION TOTTORI UNIVERSITY
  • Inventor
  • TOMITA, Motonori
IPC(International Patent Classification)
Specified countries National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IS KE KG KM KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG
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