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METHOD FOR IDENTIFYING AND QUANTIFYING TYPES OF ANIMAL HAIR

外国特許コード F140007868
整理番号 S2012-0616-C0
掲載日 2014年5月15日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2013JP061345
国際公開番号 WO 2013154208
国際出願日 平成25年4月10日(2013.4.10)
国際公開日 平成25年10月17日(2013.10.17)
優先権データ
  • 特願2012-089662 (2012.4.10) JP
発明の名称 (英語) METHOD FOR IDENTIFYING AND QUANTIFYING TYPES OF ANIMAL HAIR
発明の概要(英語) The present invention addresses the issue of providing a method which enables the identification and quantification of types of animal hair from a sample containing animal hair by directly analyzing protein which is the main component of animal hair, rather than using DNA which is present in small quantities. The present invention provides a method in which mass spectrometry of an animal hair sample is carried out, enabling, as a result of the analysis, the mass, mass/charge ratio (m/z), LC elution time for the mass/charge ratio, and a partial sequence useful as a marker to be identified for protein components such as keratin which are the main protein components of animal hair, and types of animal hair to be identified and quantified on the basis of these results.
従来技術、競合技術の概要(英語) BACKGROUND ART
Cashmere fibers are among the animal hair products, severe natural environment and were taken from the goats for cashmere, has excellent properties such as specific, and high value transactions. However, recently cashmere fibers to mix the animal fiber other disguised quality are generated, which is a problem. With respect to these display clothing, threads or fibers as test methods for the identification of the fibers, according to Japanese Industrial Standards JIS L 1030 by (Method for mixed use of the fiber product), microscopy using the specified test method. In microscopy, as the key for distinguishing the appearance of the hair and the change in the fiber surface by processing the surface of the case to resemble the appearance of the cashmere, may be affected by the identification result. In particular, yak hair brown cashmere closely resembles the shape and surface of the fiber, and cashmere 100% displayed in the product into the hair yaks, a mixed in the mixed hair yaks microscopy an identification is difficult, a more reliable and simple mixing of the bristles into the cashmere yaks method of verification is demanded. Microscopy in addition to the discrimination by the skill of a designer is an identification of the fluctuation is also a possibility. A plurality of persons is usually performed by the paper currency verification is carried out in a reliable discrimination, microscopy interpolation technique as a result the development of methods of verification or fineness was important problem. Discrimination by microscopy techniques as interpolation technique, a method of discrimination by the DNA in recent years have been reported. In particular for the identification of the article of cashmere, Society Japan spinning inspection application number: Japanese Patent Application 2003-297332 (Patent Document 1: Japanese Patent Application Laid-open 2004-121229), Application Society Japan chemical fiber inspection: Japanese 11-15616 (Patent Document 2: Japanese Patent Application Laid-open 2000-210084), application number: Japanese 11-135993 (Patent Document 3: Japanese Patent Application Laid-open 2000-325098) has been made of patent publications such as, application number: Japanese Patent being assessed for 11-15616 (Patent Document 4: patent Publication 3566570). These methods, the presence of a large number of mitochondrial DNA per cell, for example, the cytochrome b based on the sequences of primers were designed to a species-specific DNA, DNA extracted from animal hair product as a template in PCR to amplify the DNA, such as DNA amplification by analyzing the molecular weight of the species are determined. However the present inventors in the development of identification by the DNA has been switched to, in PCR, a criterion of the presence or absence of the position to be identified, and when the amplification from an animal hair may not be amplified depending on the product. Including fibers of hair roots is taken is relatively easy to DNA analysis, hair fiber is free of the hair has been mown, DNA analysis is difficult, so the industry has been mown does not contain the hair fibers is used in most cases. In addition, for such dyes, DNA is not extracted efficiently considered. In addition, for the purpose of amplifying a very small number of DNA, there is also a risk of false positives, in addition, in practice, the problem is difficult to quantify.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • GIFU UNIVERSITY
  • KE'KEN TEXTILE TESTING AND CERTIFICATION CENTER
  • 発明者(英語)
  • NISHIKAWA, Kazuya
  • YOKOGAWA, Takashi
  • OHNO, Satoshi
  • HAYASHI, Katsumasa
  • MATSUNAGA, Nobuyoshi
  • MOTOYAMA, Aya
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IS JP KE KG KM KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG
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