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DNA VACCINE AGAINST PSEUDOTUBERCULOSIS IN MARINE FISH

外国特許コード F140008007
整理番号 S2012-1123-C0
掲載日 2014年10月30日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2013JP074075
国際公開番号 WO 2014038662
国際出願日 平成25年9月6日(2013.9.6)
国際公開日 平成26年3月13日(2014.3.13)
優先権データ
  • 特願2012-198719 (2012.9.10) JP
発明の名称 (英語) DNA VACCINE AGAINST PSEUDOTUBERCULOSIS IN MARINE FISH
発明の概要(英語) Provided is a DNA vaccine for use in fish which induces protective immunity against pseudotuberculosis. As the active component, this DNA vaccine contains DNA containing a nucleotide sequence that encodes a polypeptide that is immunogenic against Photobacterium damselae subsp. piscicida, or contains an expression vector containing said DNA.
従来技術、競合技術の概要(英語) BACKGROUND ART
Representative fish and shellfish aquaculture industry many aquatic organisms, a closed system aquaculture region of the viral disease and bacterial disease is, from the fact that the individual is present at a high density, the greater the influence of their infection, the culture becomes a serious problem in the industry.
Disease include nodules (or pasteurellosis) is, in the year 1963 (Chesapeake) white perch america chesapeake Gulf (rosin residue, synchronous America: Roccus americanus) for the first time as a large amount of the cause of death disease (non-patent document 1) have been reported. South-west Shikoku Japan 0 year 1968 years its occurrence in the culture of the debris found in fish, the culture of the following year in the western part of the year 1969 epidemic at almost no debris. In addition, not only for the debris black sea bream, sea bream, kijihata, ayu, such as and also occurs in a fish species, this disease have very strong infectivity in question from the threatening marine aquacultural (non-patent document 1).
The photo P. damselae subsp. piscicida belonging to genus bacteria, gram-negative, non-motile anaerobic bacilli exhibit short penetration (0.6-1.2x0.8-2.6 µm) in one family. 25-30 ° C. is appropriate for the growth of bacteria of the present invention, the optimal pH is 7.5-8. 0, 2-3% is optimal in a concentration of sodium chloride, ampicillin, oxolinic acid, insensitive to such as florfenicol. Present the symptoms of, before and after 1 mm in the spleen and kidney formation characterized by small white spots. Small white spot is arranged on the colonies of bacteria, many nodules surrounded by fibrous tissue forms. The formation of these bacteria to colonize on the withstand intracellular digestion by phagocytic cells, phagocytic cells within the capillaries and can be caused to proliferate within the interstitial tissue and cells form spheres (non-patent document 2) based on.
In regard to, the present disease have already been approved for an inactivated vaccine is, inactivated vaccines are difficult to induction of cellular immunity. However, the ability to induce cellular immunity is hardly developed vaccine (patent document 1 and patent document 2) does not.
Is for the prevention or treatment of bacterial infections, the vaccine is generally used. Inactivated vaccines (Japanese encephalitis, such as weil's disease), toxoid (tetanus, diphtheria and the like), attenuated vaccine (BCG, such as poliovirus), gene recombinant vaccine (such as a hepatitis virus B) and the like. And an inactivated vaccine and a detoxified exotoxin toxoid, to induce antibodies against these relatively safe vaccine. The recombinant vaccine is, compared to an inactivated vaccine, because it does not contain impurities, believed to be a safe vaccine than.
However, these vaccines can induce antibody production is in is, induced cell-mediated immunity has the drawback of difficult. In addition, the vaccine is an inactivated vaccine and attenuated, industrially protein antigen is obtained in a large amount is required, suitable pathogens is essential for growth. Further, attenuated vaccine that is acquired by the immune effector is, in many cases is maintained for a long period, side effects on the other hand, there is a risk that pointed out. And genetically engineered vaccine is an inactivated vaccine, the persistence of the antigen and shorter in the host, in need of adjuvant. These conventional type of vaccine from manufacture until inoculation to a subject, it is necessary to refrigerated storage, the increase in cost and a problem occurs that the decreased potency of.
Recently, vaccine research and development proceeds, the immunogenic protein by administration of a plasmid encoding DNA, one immune induction of a new vaccine (DNA vaccine) have been developed, the disadvantage of a conventional type such as vaccine described below have been improved. That is, DNA vaccine, as well as a humoral immune response, can be induced strong cell-mediated immunity, to confer protection from infection can be, further, can be highly purified, stable even under high temperature or room temperature, refrigerated storage is not essential that the long term storage is possible, by a genetic engineering procedure easy rapid improvement DNA vaccine, and shortening of the time spent by vaccine development and advantages.
Constituent proteins of rhabdoviruses (Rhabdovirus) of glycoprotein encoding gene by injection into the muscle, soleus of rainbow trout and stimulate an immune response is known (non-patent document 3). In addition, the vaccine for rainbow trout and soleus DNA (non-patent document 4) a report. However, in other fish species no report of vaccine DNA.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • NATIONAL UNIVERSITY CORPORATION TOKYO UNIVERSITY OF MARINE SCIENCE AND TECHNOLOGY
  • 発明者(英語)
  • HIRONO Ikuo
  • KONDO Hidehiro
  • YAMASHITA Kozue
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IS JP KE KG KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN TD TG

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