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GLUCOSYL-α-1,2-GLYCEROL PHOSPHORYLASE AND METHOD FOR PRODUCING GLUCOSYL-α-1,2-GLYCEROL USING SAME 新技術説明会

外国特許コード F140008023
整理番号 S2013-0286-C0
掲載日 2014年11月18日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2013JP082768
国際公開番号 WO 2014103659
国際出願日 平成25年12月6日(2013.12.6)
国際公開日 平成26年7月3日(2014.7.3)
優先権データ
  • 特願2012-286731 (2012.12.28) JP
発明の名称 (英語) GLUCOSYL-α-1,2-GLYCEROL PHOSPHORYLASE AND METHOD FOR PRODUCING GLUCOSYL-α-1,2-GLYCEROL USING SAME 新技術説明会
発明の概要(英語) Provided are a novel glucosyl-α-1,2-glycerol phosphorylase that makes it possible to mass-produce glucosyl-α-1,2-glycerol easily and selectively from inexpensive materials, and a method for producing glucosyl-α-1,2-glycerol using the same. According to the present invention, glucosyl-α-1,2-glycerol is mass-produced easily and selectively by (1) conducting an enzymatic reaction in a solution containing β-glucose 1-phosphate, glycerol, and glucosyl-α-1,2-glycerol phosphorylase or (2) conducting an enzymatic reaction in a solution containing a carbohydrate phosphorolytic enzyme that produces β-glucose 1-phosphate, and a sugar that serves as a substrate thereof, and glycerol, phosphoric acid, and glucosyl-α-1,2-glycerol phosphorylase.
従来技術、競合技術の概要(英語) BACKGROUND ART
Traditional Brewing Society of Japan and the fermented soybean paste, sweet sake have shown that the glucosyl - α - 1, glycerol is 2 -, the action to suppress cancer cell growth, anti-allergic action upon, vascular endothelial cell growth promoting factor production promoting action, blood glucose increase inhibitory effect, antibacterial effect, cell energizing action, dermal matrix production promoting action, inhibitory effect on neutral fat accumulation, melanin production suppressing, have been reported and the reduction effect of skin irritation, food, cosmetic, pharmaceuticals are expected to be used as a raw material (Patent Document 1 and 2). In this way as well as from the surface of the nutrient in recent years, the functionality of the oligosaccharides are of interest, the difficulty of preparing high purity, the high production cost hamper the industrial application of the study. Therefore, functional oligosaccharides of up to and including a variety of useful selective low-cost mass preparation method the currently established has been strongly requested.
However, conventional glucosyl - α - 1, glycerol production processes include 2 -, and the following drawbacks, could not be produced at a low cost. Chemical oxidation method is, using the periodate oxidation reaction, can be synthesized from the maltitol is, the position selectivity of the reaction is not sufficient, the yield was low (18%: weight ratio). In addition, the enzymatic synthesis method, sucrose phosphorylase and α - glucosidase can be synthesized by the reaction of the glycoproteins, reaction itself is not the original reaction of these enzymes, excess glycerol must be carried out in the presence, further α - glucosidase is used as the byproducts from the reaction site for selective, and had a low yield (5 .9%: glycerol mole ratio on the basis). In addition, a problem that disassembly of the synthesis products and the like.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • NIIGATA UNIVERSITY
  • 発明者(英語)
  • NAKAI, Hiroyuki
  • NIHIRA, Takanori
  • SAITO, Yuka
  • OHTSUBO, Kenichi
  • KITAOKA, Motomitsu
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS KE KG KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN TD TG
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