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NOVEL IRON-ZINC BINDING CONTROL FACTOR, AND TECHNIQUE FOR IMPROVING IRON DEFICIENCY TOLERANCE OF PLANT AND ENHANCING IRON AND ZINC ACCUMULATION IN EDIBLE PART THEREOF BY CONTROLLING EXPRESSION OF NOVEL IRON-ZINC BINDING CONTROL FACTOR

Foreign code F150008145
File No. K20101WO
Posted date Mar 17, 2015
Country WIPO
International application number 2013JP069628
International publication number WO 2014017394
Date of international filing Jul 19, 2013
Date of international publication Jan 30, 2014
Priority data
  • P2012-166233 (Jul 26, 2012) JP
Title NOVEL IRON-ZINC BINDING CONTROL FACTOR, AND TECHNIQUE FOR IMPROVING IRON DEFICIENCY TOLERANCE OF PLANT AND ENHANCING IRON AND ZINC ACCUMULATION IN EDIBLE PART THEREOF BY CONTROLLING EXPRESSION OF NOVEL IRON-ZINC BINDING CONTROL FACTOR
Abstract The present invention provides: a transformant and a gene-disrupted strain which grow better in a calcareous soil than common plants and can accumulate iron and zinc in a larger amount both in a calcareous soil and in a good soil; a gene, a vector, a protein and an antibody to be used for constructing the aforesaid transformant and gene-disrupted strain; a method for constructing a plant that has improved tolerance to iron deficiency and shows enhanced iron and zinc accumulation in an edible part thereof; a composition for constructing the aforesaid plant; a kit for constructing the aforesaid plant; and a breeding method. The protein according to the present invention, which comprises one of the following amino acids (a) to (c), is characterized by being an iron-zinc binding control factor: (a) an amino acid sequence represented by SEQ ID NO:1 or 2; (b) an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO:1 or 2 by deletion, substitution or addition of one to several amino acids; and (c) an amino acid sequence that has an identity of 80% or greater with the amino acid sequence represented by SEQ ID NO:1 or 2.
Outline of related art and contending technology BACKGROUND ART
The plant is growing, in order to perform the substance production and carbon fixation, iron and zinc is required. Plants, the soil due to the absorption of iron and zinc, these are used. However, approximately 30% of the soil in the world occupies in calcareous alkaline soil, solubilized iron and zinc is small, even in the solubilized iron is extremely small. Thus, growth of the plants in the soil of the calcareous alkali, iron deficiency is a major limiting factor for. Therefore, calcareous alkaline soil failure including the acquisition of the plants growing in soil is improved is an urgent need.
In addition, iron and zinc from the soil and the plants that were absorbed, human major mineral source. Iron deficiency and zinc deficiency is, people in the world, especially in children and therefore becomes a serious problem for women, edible part of plants containing a large amount of iron and zinc acquisition has been desired.
In recent years, iron and zinc absorption and use (especially iron) the identification of genes involved in advanced and analysis. A modification of the gene by introducing into the plant, iron and zinc deficiency resistance is improved on the plant, or, a large number of edible plant to accumulate iron and zinc are acquired (for example, see non-patent document 1-11).
Scope of claims (In Japanese)請求の範囲 [請求項1]
 以下の(a)~(c)のいずれか一つのアミノ酸配列からなり、かつ鉄・亜鉛結合性制御因子であることを特徴とするタンパク質。
(a)配列番号1又は2で表されるアミノ酸配列、
(b)配列番号1又は2で表されるアミノ酸配列において、1~数個のアミノ酸が欠失、置換又は付加されているアミノ酸配列、又は、
(c)配列番号1又は2で表されるアミノ酸配列と同一性が80%以上であるアミノ酸配列

[請求項2]
 請求項1に記載のタンパク質をコードすることを特徴とする遺伝子。

[請求項3]
 以下の(d)~(g)のいずれか一つのDNAからなり、かつ鉄・亜鉛結合性制御因子であるタンパク質をコードすることを特徴とする遺伝子。
(d)配列番号3又は4で表される塩基配列からなるDNA、
(e)配列番号3又は4で表される塩基配列において、1~数個の塩基が欠失、置換又は付加されている塩基配列からなるDNA、
(f)配列番号3又は4で表される塩基配列と同一性が80%以上である塩基配列からなるDNA、又は、
(g)配列番号3又は4で表される塩基配列からなるDNAと相補的な塩基配列からなるDNAとストリンジェントな条件下でハイブリダイズすることができる塩基配列からなるDNA

[請求項4]
 請求項2又は3に記載の遺伝子の発現を抑制し得ることを特徴とするベクター。

[請求項5]
 前記遺伝子の発現をmRNAレベルで抑制し得るRNAi誘導性核酸を発現可能である請求項4に記載のベクター。

[請求項6]
 前記RNAi誘導性核酸が、配列番号5で表される塩基配列である請求項5に記載のベクター。

[請求項7]
 請求項4~6のいずれか一項に記載のベクターを宿主に導入してなることを特徴とする形質転換体。

[請求項8]
 挿入配列が組み込まれることにより、請求項2又は3に記載の遺伝子が破壊されているゲノムDNAを有することを特徴とする遺伝子破壊株。

[請求項9]
 請求項1に記載のタンパク質と特異的に結合することを特徴とする抗体。

[請求項10]
 請求項4~6のいずれか一項に記載のベクターを植物に導入する工程を含むことを特徴とする鉄欠乏耐性及び可食部への鉄・亜鉛蓄積が向上した植物の作出方法。

[請求項11]
 請求項4~6のいずれか一項に記載のベクターを備えていることを特徴とする鉄欠乏耐性及び可食部への鉄・亜鉛蓄積が向上した植物の作出用組成物。

[請求項12]
 請求項4~6のいずれか一項に記載のベクターを備えていることを特徴とする鉄欠乏耐性及び可食部への鉄・亜鉛蓄積が向上した植物の作出用キット。

[請求項13]
 植物からの抽出液に含まれる請求項1に記載のタンパク質を検出する工程を含むことを特徴とする鉄欠乏耐性及び可食部への鉄・亜鉛蓄積が向上した植物の育種方法。

[請求項14]
 植物からの抽出液に含まれる請求項2又は3に記載の遺伝子を検出する工程を含むことを特徴とする鉄欠乏耐性及び可食部への鉄・亜鉛蓄積が向上した植物の育種方法。

  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • Inventor
  • KOBAYASHI Takanori
  • NISHIZAWA Naoko
IPC(International Patent Classification)
Specified countries National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IS JP KE KG KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN TD TG
Reference ( R and D project ) PRESTO Creation of essential technologies to utilize carbon dioxide as a resource through the enhancement of plant productivity and the exploitation of plant products AREA
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