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AGROBACTERIUM HAVING HIGHLY EFFICIENT GENE TRANSFER ABILITY TO PLANT IMPARTED THERETO UPDATE

外国特許コード F150008206
掲載日 2015年3月25日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2014JP052844
国際公開番号 WO 2014123208
国際出願日 平成26年2月7日(2014.2.7)
国際公開日 平成26年8月14日(2014.8.14)
優先権データ
  • 特願2013-023726 (2013.2.8) JP
発明の名称 (英語) AGROBACTERIUM HAVING HIGHLY EFFICIENT GENE TRANSFER ABILITY TO PLANT IMPARTED THERETO UPDATE
発明の概要(英語) An agrobacterium showing an improved gene transfer efficiency. Provided are a transformed agrobacterium that holds a foreign GABA transaminase gene and shows an improved gene transfer efficiency, and a method for producing a transformed plant using the same.
従来技術、競合技術の概要(英語) BACKGROUND ART
One of the soil bacteria in a plant via Agrobacterium bacteria (Agrobacterium method) is a gene introducing method, without the need for a special technique or device can be carried out in a simple procedure. In Agrobacterium method, gene copy numbers and with a smaller complete form can be introduced in the plant. For this reason, compared to other plant gene transfer methods, transformed plants used in the production of the high frequency. However, low efficiency introduction by Agrobacterium method there are a multitude of plant species. Therefore the ability of gene transfer into plant Agrobacterium development has been desired.
The ability to inhibit ethylene production Agrobacterium bacteria ACC deaminase gene and the plasmid containing the applied by holding a, to improve the efficiency of gene transfer into plant Agrobacterium bacteria for (super agrobacterium) have been reported (patent document 1, non-patent document 1). However based on various mechanisms in order to improve the gene introduction efficiency, the development of high gene transfer efficiency into Agrobacterium bacteria is still desired. The inventors of the present invention, a predetermined area of the fungal genomic Agrobacterium by incorporating ACC deaminase gene, the bacteria Agrobacterium to stably increase the gene transfer efficiency into have developed a technique (patent document 2).
However, plant GABA (γ - amino butyric acid) or injury to the plants are produced in the same Agrobacterium infections (non-patent document 2). In addition, high-producing plants are GABA Agrobacterium, infection for strain stably (Agrobacterium tumefaciens) C58 were less susceptible (non-patent document 3) has been a report that. Further, quorum sensing signal plant GABA Agrobacterium bacteria is suppressed, and to the introduction of genes necessary for plasmid replication Ti, between the horizontal for suppressing propagation of bacteria Agrobacterium is known (non-patent document 4 and 5). However plant GABA activity, can be changed by the modification of Agrobacterium bacteria whether or not, and hence the gene introduction efficiency can be whether the change is not known.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • UNIVERSITY OF TSUKUBA
  • 発明者(英語)
  • EZURA Hiroshi
  • NAKAMURA Koji
  • NONAKA Satoko
  • SOMEYA Tatsuhiko
  • ZHOU Sha
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN TD TG
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