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AGROBACTERIUM HAVING HIGHLY EFFICIENT GENE TRANSFER ABILITY TO PLANT IMPARTED THERETO meetings

Foreign code F150008206
Posted date Mar 25, 2015
Country WIPO
International application number 2014JP052844
International publication number WO 2014123208
Date of international filing Feb 7, 2014
Date of international publication Aug 14, 2014
Priority data
  • P2013-023726 (Feb 8, 2013) JP
Title AGROBACTERIUM HAVING HIGHLY EFFICIENT GENE TRANSFER ABILITY TO PLANT IMPARTED THERETO meetings
Abstract An agrobacterium showing an improved gene transfer efficiency. Provided are a transformed agrobacterium that holds a foreign GABA transaminase gene and shows an improved gene transfer efficiency, and a method for producing a transformed plant using the same.
Outline of related art and contending technology BACKGROUND ART
One of the soil bacteria in a plant via Agrobacterium bacteria (Agrobacterium method) is a gene introducing method, without the need for a special technique or device can be carried out in a simple procedure. In Agrobacterium method, gene copy numbers and with a smaller complete form can be introduced in the plant. For this reason, compared to other plant gene transfer methods, transformed plants used in the production of the high frequency. However, low efficiency introduction by Agrobacterium method there are a multitude of plant species. Therefore the ability of gene transfer into plant Agrobacterium development has been desired.
The ability to inhibit ethylene production Agrobacterium bacteria ACC deaminase gene and the plasmid containing the applied by holding a, to improve the efficiency of gene transfer into plant Agrobacterium bacteria for (super agrobacterium) have been reported (patent document 1, non-patent document 1). However based on various mechanisms in order to improve the gene introduction efficiency, the development of high gene transfer efficiency into Agrobacterium bacteria is still desired. The inventors of the present invention, a predetermined area of the fungal genomic Agrobacterium by incorporating ACC deaminase gene, the bacteria Agrobacterium to stably increase the gene transfer efficiency into have developed a technique (patent document 2).
However, plant GABA (γ - amino butyric acid) or injury to the plants are produced in the same Agrobacterium infections (non-patent document 2). In addition, high-producing plants are GABA Agrobacterium, infection for strain stably (Agrobacterium tumefaciens) C58 were less susceptible (non-patent document 3) has been a report that. Further, quorum sensing signal plant GABA Agrobacterium bacteria is suppressed, and to the introduction of genes necessary for plasmid replication Ti, between the horizontal for suppressing propagation of bacteria Agrobacterium is known (non-patent document 4 and 5). However plant GABA activity, can be changed by the modification of Agrobacterium bacteria whether or not, and hence the gene introduction efficiency can be whether the change is not known.
Scope of claims (In Japanese)請求の範囲 [請求項1]
 外来性のGABAアミノ基転移酵素遺伝子を保持する、向上した遺伝子導入効率を示す形質転換アグロバクテリウム菌。

[請求項2]
 外来性のACCデアミナーゼ遺伝子をさらに保持する、請求項1に記載の形質転換アグロバクテリウム菌。

[請求項3]
 GABAアミノ基転移酵素遺伝子及び/又はACCデアミナーゼ遺伝子をベクター形態で保持する、請求項1又は2に記載の形質転換アグロバクテリウム菌。

[請求項4]
 T-DNA領域を含むバイナリーベクターをさらに含有する、請求項1~3のいずれか1項に記載の形質転換アグロバクテリウム菌。

[請求項5]
 GABAアミノ基転移酵素遺伝子が細菌由来である、請求項1~4のいずれか1項に記載の形質転換アグロバクテリウム菌。

[請求項6]
 ACCデアミナーゼ遺伝子が細菌由来である、請求項1~5のいずれか1項に記載の形質転換アグロバクテリウム菌。

[請求項7]
 請求項1~6のいずれか1項に記載の形質転換アグロバクテリウム菌を用いて、植物への遺伝子導入を行うことを特徴とする、形質転換植物の作製方法。

[請求項8]
 植物が単子葉植物又は双子葉植物である、請求項7に記載の方法。

[請求項9]
 単子葉植物がイネ科植物である、請求項8に記載の方法。

[請求項10]
 双子葉植物がナス科植物である、請求項8に記載の方法。

  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • UNIVERSITY OF TSUKUBA
  • Inventor
  • EZURA Hiroshi
  • NAKAMURA Koji
  • NONAKA Satoko
  • SOMEYA Tatsuhiko
  • ZHOU Sha
IPC(International Patent Classification)
Specified countries National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN TD TG
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