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BIOMARKER FOR TESTING POSSIBILITY OF FERTILIZATION OF OVUM AND DETERMINATION USING SAME

Foreign code F150008253
Posted date Mar 27, 2015
Country WIPO
International application number 2014JP068506
International publication number WO 2015016036
Date of international filing Jul 10, 2014
Date of international publication Feb 5, 2015
Priority data
  • P2013-156672 (Jul 29, 2013) JP
Title BIOMARKER FOR TESTING POSSIBILITY OF FERTILIZATION OF OVUM AND DETERMINATION USING SAME
Abstract Provided is a novel biomarker for testing the possibility of fertilization of an ovum. The method for testing the possibility of fertilization of an ovum in a subject is characterized by comprising measuring the amount of one or more peptides selected from the peptides of the amino acid sequences represented by SEQ ID NOS:1 to 4 in a follicular fluid that is collected from the subject.
Outline of related art and contending technology BACKGROUND ART
Next in Japan is declining birthrate elderly, prenatal human 1 human 50, about 2 million per year is worn by the child and in-vitro fertilization, declining birthrate and aging of the pawl in-vitro fertilization is essential to the medical personnel in recent years as it has been widely used. On the other hand, the success rate of in-vitro fertilization, such as fertility, depending on the state of the egg protein or collecting However, the quality or state suitable for fertilization ovae is currently no method to determine.
Conventionally, after the processing of in-vitro fertilization of the ovum by the microscopic observation of the physician was a selection method, in this case, depending on the experience of the surgeons, together with the entering subjective judgment, determination takes a long time.
According to a recent study, in accordance with a change in the quality of ova in the elderly, illnesses that pregnancy, childbirth and the high risk factor. At the time of in-vitro fertilization when harvesting ovae obtained at the same time from a component of follicular fluid, such as fertility attempting to determine the quality of ova attempts have been made (Patent Document 1 and Non-Patent Document 1), can be examined quickly with high precision suitable biomarker has not been discovered until now.
In-vitro fertilization in the future to further increase the ratio of the old child is predicted from, the success rate of fertilization and a problem of increase of the urgent. Ovae high fertility or selection method of determining a developed which, leads to an improvement of the success rate of in-vitro fertilization, and is coupled to the desired good news for a child, the progress in the future are expected to be applied to the declining birthrate than Japan pawls elderly that may be one of the means of the expected.
Scope of claims (In Japanese)請求の範囲 [請求項1]
 被検者より採取した卵胞液中の、配列番号1~4で表されるアミノ酸配列からなるペプチド群より選択される1以上のペプチドの量を測定することを特徴とする、該被検者における卵子の受精可能性の検査方法。

[請求項2]
 卵胞液を質量分析にかけることを含む、請求項1に記載の方法。

[請求項3]
 前記測定により得られたペプチドの量を、対照試料中のペプチドの量に基づいて設定された閾値と比較し、前記測定により得られたペプチドの量が対照試料中のペプチドの量に基づいて設定された閾値に比べて統計学的に有意に変動している場合に、卵子の受精可能性が高いと判定することをさらに含む、請求項1に記載の方法。

[請求項4]
 配列番号1~4で表されるアミノ酸配列からなるペプチド群より選択されるペプチドを含む卵子の受精可能性の検査のためのバイオマーカー。

[請求項5]
 配列番号1~4で表されるアミノ酸配列からなるペプチド群より選択されるペプチドからなる卵子の受精可能性の検査のためのバイオマーカー。

[請求項6]
 卵子の受精可能性を検査する方法であって、
 配列番号1~4で表されるアミノ酸配列からなるペプチド群より選択されるペプチドを特異的に認識する抗体を用いた方法。

[請求項7]
 抗体を含む卵子の受精可能性を検査するための判定キットであって、当該抗体が、配列番号1~4で表されるアミノ酸配列からなるペプチド群より選択されるペプチドを特異的に認識する抗体である、判定キット。

[請求項8]
 配列番号1~4で表されるアミノ酸配列からなるペプチド群より選択されるペプチドに対する抗体を固定した免疫診断または判定用装置。

[請求項9]
 配列番号1、配列番号2、配列番号3、または配列番号4で表されるアミノ酸配列からなるペプチド。

  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • NATIONAL UNIVERSITY CORPORATION UNIVERSITY OF FUKUI
  • PROTOSERA INC.
  • Inventor
  • MIYAMOTO, Kaoru
  • MIZUTANI, Tetsuya
  • ORISAKA, Makoto
  • TANAKA, Kenji
  • LEE, Lyang-Ja
  • NONAKA, Daisuke
  • ASADA, Kyoichi
IPC(International Patent Classification)
Specified countries National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN TD TG
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