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CRYSTALLIZATION-PROMOTING POLYPEPTIDE UPDATE 新技術説明会

外国特許コード F150008266
掲載日 2015年3月31日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2014JP062215
国際公開番号 WO 2014181786
国際出願日 平成26年5月7日(2014.5.7)
国際公開日 平成26年11月13日(2014.11.13)
優先権データ
  • 特願2013-097698 (2013.5.7) JP
発明の名称 (英語) CRYSTALLIZATION-PROMOTING POLYPEPTIDE UPDATE 新技術説明会
発明の概要(英語) The purpose of the present invention is to provide a versatile and effective method for promoting the crystallization of a protein of interest in X-ray crystal structure analysis. The abovementioned problem can be solved by (1) a polypeptide comprising the amino acid sequence represented by SEQ ID NO:1, (2) a polypeptide comprising the amino acid sequence represented by SEQ ID NO:2, and (3) a polypeptide comprising the amino acid sequence represented by SEQ ID NO:2, wherein 1 to 18 amino acids have been deleted from the N-terminal side, and/or any of 1 to 3 amino acids have been deleted from the C-terminal side.
従来技術、競合技術の概要(英語) BACKGROUND ART
The structural analysis of protein X-ray crystal structure analysis method, NMR method, or electron microscope analysis is mainly used. In which, X-ray crystal structure analysis method, an electron microscope image can be obtained with higher accuracy than molecules. In addition, as compared to large molecules NMR in molecular weight can also be applied. Therefore, X-ray crystal structure analysis method, as a method for structural analysis of protein, a general-purpose. However, X-ray crystal structure analysis method for analysis of proteins, protein crystal must be made. In the single molecule protein, due to low weak X-ray scattering, the analysis is not possible. Protein crystals, since the diffraction light is amplified, scattering intensity can be measured, by calculating the electron density it, to create a model structure of the protein molecule becomes possible.
However, the crystallization of the protein X-ray crystal structure analysis method is also the largest barrier. Protein crystallization conditions, for example even between proteins belonging to the same family are greatly different from, and precipitation pH is very sensitive to changes of the concentration. In addition, crystallisation of proteins is not seen in current mechanism. Therefore, the crystallization of the protein, and a large amount of conditions necessary to conduct a study, a large amount of labor, time, and costly. Then, in the X-ray crystal structure analysis method, for good-quality crystal cannot be obtained, often cannot be protein structure analysis. The use of a protein X-ray crystal structure analysis in order to obtain, for examining the condition of the large number of crystal kit developed crystallization, crystallization conditions to be used in the study was conducted to the robot. Further, a zero-gravity field (space) the crystallization, the crystallization by irradiation with laser or magnetic field but have been examined, and both say that sufficient effect was unacceptable.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY
  • 発明者(英語)
  • YAO Min
  • KOMODA Keisuke
  • YU Jian
  • TANAKA Yoshikazu
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN TD TG
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