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Peptide having affinity for silicon nitride (Si3N4), and use therefor meetings

Foreign code F150008350
File No. 外0102-2
Posted date May 20, 2015
Country United States of America
Application number 201314378433
Gazette No. 20150038675
Gazette No. 9701760
Date of filing Feb 12, 2013
Gazette Date Feb 5, 2015
Gazette Date Jul 11, 2017
International application number JP2013053290
International publication number WO2013122061
Date of international filing Feb 12, 2013
Date of international publication Aug 22, 2013
Priority data
  • P2012-028681 (Feb 13, 2012) JP
  • 2013WO-JP53290 (Feb 12, 2013) WO
Title Peptide having affinity for silicon nitride (Si3N4), and use therefor meetings
Abstract (US9701760)
The purpose of the present invention is to provide: a peptide having an affinity for silicon nitride; a polynucleotide encoding the peptide; an expression vector for expressing the peptide having an affinity for silicon nitride; an expression vector for expressing a peptide fusion protein that comprises the peptide having an affinity for silicon nitride and a target protein; a transformant obtained by introducing the expression vector into a host cell; a peptide fusion protein obtained from the transformant; a silicon nitride substrate to which a peptide having an affinity for silicon nitride has been bonded; a method for immobilizing a target protein to a silicon nitride substrate; a composition for immobilizing a target protein to a silicon nitride substrate, the composition comprising a peptide having an affinity for silicon nitride; and a linker for immobilizing a target protein to a silicon nitride substrate, the linker comprising a peptide having an affinity for silicon nitride.
The invention involves a peptide having an affinity for silicon nitride, the peptide comprising (1-1) a peptide having the amino acid sequence of one of SEQ ID NOS: 1, 2 and 23 to 27, (1-2) a peptide that has an affinity for silicon nitride and comprises an amino acid sequence obtained by deleting, adding, and/or substituting one or more amino acids in one of the amino acid sequences indicated in (1-1), or a fragment of one of the peptides.
Scope of claims [claim1]
1. A silicon nitride substrate comprising a peptide having an affinity for silicon nitride bonded thereto, the peptide consisting of any one of SEQ ID NOS: 1 to 11.
[claim2]
2. A silicon nitride substrate comprising a target protein immobilized to the silicon nitride substrate via a peptide of any one of SEQ ID NOS: 1 to 11, the peptide having an affinity for silicon nitride, and the target protein selected from the group consisting of an antibody and a lectin.
[claim3]
3. A method for immobilizing a target protein to a silicon nitride substrate comprising: contacting a silicon nitride substrate with the target protein fused to a peptide having an affinity for silicon nitride, the peptide consisting of any one of SEQ ID NOS: 1 to 11, the target protein selected from the group consisting of an antibody and a lectin.
  • Inventor, and Inventor/Applicant
  • KUMADA YOICHI
  • KISHIMOTO MICHIMASA
  • OTSUKA TAKERU
  • KYOTO INSTITUTE OF TECHNOLOGY
IPC(International Patent Classification)
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