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RICE RT-TYPE CYTOPLASMIC MALE STERILITY CAUSAL GENE AND USE THEREOF

外国特許コード F150008360
掲載日 2015年6月29日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2013JP066528
国際公開番号 WO 2014027502
国際出願日 平成25年6月10日(2013.6.10)
国際公開日 平成26年2月20日(2014.2.20)
優先権データ
  • 特願2012-180824 (2012.8.17) JP
発明の名称 (英語) RICE RT-TYPE CYTOPLASMIC MALE STERILITY CAUSAL GENE AND USE THEREOF
発明の概要(英語) The purpose of the present invention is to identify the causal gene of rice RT-type cytoplasmic male sterility and to provide a means for determining a rice sterile line that possesses RT-type male-sterile cytoplasm by using said causal gene as a DNA marker, in order to employ rice RT-type cytoplasmic male sterility in a three-line method. The present invention provides: a causal gene of rice RT-type cytoplasmic male sterility that includes a DNA comprising a base sequence represented by SEQ ID NO: 1 or 3; and a method for determining a rice sterile line that possesses RT-type male-sterile cytoplasm by detecting the presence of said gene in a rice to be tested.
従来技術、競合技術の概要(英語) BACKGROUND ART
One generation hybrid breeding method is also referred to as hybrid cultivar breeding method, in accordance with the parents have excellent traits, and, in the hybrid varieties can be grown for bias, used in a variety of plant. Hybrid rice in rice (one margin hybrid varieties) for breeding, cytoplasmic male sterility (CMS: Cytoplasmic Male Sterility) of three-line system is used. Cytoplasmic male sterility is, by the operation of the abnormal mitochondrial gene, a phenomenon that interfere with the formation of pollen. To the mitochondrion of the cytoplasmic male sterility gene responsible for male sterility is present, on the other hand, the fertility restoration gene with a nuclear-encoded modified chimeric gene product is performed to avoid the inhibition believed. Is a three-line system, carrying cytoplasmic male sterility system for the 'male sterile line', carrying a fertility restoration gene with a single system (Rf) 'fertility recovery system', and, nuclear genes is identical to the male sterile line which do not have cytoplasmic male sterile system for the 'maintain system' refers to using breeding methods. Using these 3 system, the recovery system (i) pollen from male sterile line by mating of the hybrid seed can be obtained, (ii) maintaining the male sterile line by crossing with pollen of the system to maintain the male sterile line, are able to grow. In the three-line system utilizing cytoplasmic male sterility can be carried out, cytoplasmic male sterility reliably whether the rice male sterile line to hold the assay is required. Conventional, plant cytoplasmic male sterility in order to determine the genotype of, first, and positive and negative test crossing and self-pollinated, seed formation rate to investigate the frequency of appearance of individuals 0% is necessary. However, this method requires much time and year required much labor is required, in addition, the temperature and duration of sunshine seed fertility may easily affected by environmental conditions such as the assay cannot be accurately. Therefore, efficiently rapid and easy identification of cytoplasmic male sterility rice is a method for assaying has been desired. Compared with a conventional three-line system hybrid rice varieties using the rice variety is grown Chinsurah Boro II India BT-type cytoplasmic male sterility or wild rice varieties of hainan island WA-type cytoplasmic male sterility has been used, the main flow WA-type cytoplasmic male sterility (non-patent document 1) in. From the viewpoint of the total rice cultivation area of the world, the interrupt 1 single WA-type male sterility cytoplasm becomes dependent on the. Up to now WA-type cytoplasmic male sterility rice cytoplasmic male sterility is the cause of orf126 as a candidate for the communication of the other genes have been reported (non-patent document 2). However, cytoplasmic male sterility is directly involved in or has not been demonstrated experimentally. Therefore, WA-type cytoplasmic male sterility, cytoplasmic causal genes, fertility restoration gene with fertility of the identity of the recovery system is clearly not, molecular basis remains unsolved is being used. In addition, other than the above cytoplasmic male sterility for the system to provide a report some rice. For example, type RT98 (RT98A) cytoplasmic male sterility system is of the university of the ryukyus motomura et al., wild rice Oryza rufipogon K98 system (a system number National Laboratory W1109) and 1 times the parent, #8 in table 65 and the three consecutive backcross can be performed in a system which has grown, but apparently normal pollen, has no function of fertilization. In addition, the growth in the course, the same cytoplasmic but that RT98A, K98 having a fertility restoration gene derived from fertile fertility is restored in order to grow a RT98C recovery system (non-patent document 3) are. In addition, type RT102 (RT102A) cytoplasmic male sterility system is, of the system K102 in the same manner as Oryza rufipogon (National Laboratory system number W1125) to a parent 1 times, 8 times in one continuous backcross Nos 65 performed in a system which has grown, and the pollen does not accumulate in the starch accumulation and was observed in a mixed with, both not being capable of fertilization. In addition, the growth of course, is the same with the cytoplasm RT102A, having a fertility restoration gene derived from K102 for regenerating the recovery system RT102C is restored fertility are growing (non-patent document 4). However, type RT98 and type RT102 cytoplasmic male sterility cytoplasmic male sterility is the cause of the mitochondrial gene has not been reported. In order to use the growth of hybrid varieties, these RT-type cytoplasmic male sterility to identify genes that cause mitochondrial, cloning has been desired.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • TOHOKU UNIVERSITY
  • UNIVERSITY OF THE RYUKYUS
  • 発明者(英語)
  • TORIYAMA, KINYA
  • KAZAMA, TOMOHIKO
  • MOTOMURA, KEIJI
  • IGARASHI, KEISUKE
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IS JP KE KG KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN TD TG
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