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LIGNOCELLULOSE-DEGRADANT-RESISTANT MICROORGANISM AND METHOD FOR PRODUCING SAME, AND METHOD FOR PRODUCING BIOETHANOL USING SAID MICROORGANISM 新技術説明会

外国特許コード F150008403
掲載日 2015年7月14日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2014JP079090
国際公開番号 WO 2015079868
国際出願日 平成26年10月31日(2014.10.31)
国際公開日 平成27年6月4日(2015.6.4)
優先権データ
  • 特願2013-246294 (2013.11.28) JP
発明の名称 (英語) LIGNOCELLULOSE-DEGRADANT-RESISTANT MICROORGANISM AND METHOD FOR PRODUCING SAME, AND METHOD FOR PRODUCING BIOETHANOL USING SAID MICROORGANISM 新技術説明会
発明の概要(英語) Provided is a microorganism having resistance to a lignocellulose degradant which is a factor that inhibits the fermentation of wood. One embodiment of the present invention is a lignocellulose-degradant-resistant microorganism expressing a higher level of a gene that encodes protein (1) or (2) than does the wild type. A gene that encodes a protein represented by SEQ ID NO: 1 among these genes is a SUMO gene known as an SMT3 gene in yeast. (1) At least one protein selected from the group comprising proteins that comprise amino acid sequences represented by SEQ ID NOS: 1-4; (2) at least one protein selected from the group comprising proteins that comprise amino acid sequences in which one or several amino acids in the amino acid sequences represented by SEQ ID NOS: 1-4 have been deleted, substituted, or added, the amino acid sequences being modified within the range of 80% or higher identity with the amino acid sequence of the corresponding SEQ ID NO., and that have a function for imparting lignocellulose-degradant resistance to microorganisms.
従来技術、競合技術の概要(英語) BACKGROUND ART
An energy source with reduced load on the environment, such as wood as raw material of the chemical or herbaceous lignocellulose-based biomass additional utilizing is demanded. In order to use the lignocellulose-based biomass, these constituents are broken down into monosaccharides, the monosaccharide bioethanol as a raw material to manufacture a variety of substances such as has been studied. And more specifically, lignocellulose-based biomass, cellulose or , tightly bound to lignin structure comprises lignocellulose, for producing ethanol therefrom, to decompose the lignocellulosic once, or the like and is fermented at need. However, the lignocellulose decomposition product to be, or furfural, HMF, glycolaldehyde, such as acetic fermentation inhibitor and, the presence of the decomposed product of these which causes lowering of the fermentation efficiency was.
Such as a method for fermenting the lignocellulose, in for example Patent Document 1, particularly focusing on the step for hydrolysis of lignocellulosic processes, has been proposed in the conventional art and a method using a chemical such as an acid, such as finely divided mechanical treatment, such as high temperature and high pressure water is used as a method for solving the problems of the method, water vapor pressure of the heat treatment step such as a predetermined value and producing ethanol is disclosed.
In addition, the Patent Document 2, cellulosic biomass fuel grade ethanol is used as a raw material to use it for the efficient production, transformation of the microbe of interest derived from hemicellulose to the method for introducing the gene for the enzyme as the metabolic system of mannose is disclosed.
In addition, patent document 3 is in non-patent document 1, for the pressurized hydrothermal treatment of cellulose and glycolaldehyde as a method to improve the tolerance of yeast, expression of the ADH1 disclosed research interest.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • SAGA UNIVERSITY
  • 発明者(英語)
  • KITAGAKI, Hiroshi
  • HAYASHI, Nobuyuki
  • Lahiru Niroshan Jayakody
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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