Top > Search of International Patents > Method for detecting regulatory T cells using expression of folate receptor 4 as indicator, method for treating diseases using the detection method, pharmaceutical composition for immunostimulation, and method for treating diseases using the composition

Method for detecting regulatory T cells using expression of folate receptor 4 as indicator, method for treating diseases using the detection method, pharmaceutical composition for immunostimulation, and method for treating diseases using the composition

Foreign code F150008441
File No. 734
Posted date Oct 21, 2015
Country United States of America
Application number 24036105
Gazette No. 20060246063
Gazette No. 7488474
Date of filing Oct 3, 2005
Gazette Date Nov 2, 2006
Gazette Date Feb 10, 2009
Priority data
  • P2005-134279 (May 2, 2005) JP
Title Method for detecting regulatory T cells using expression of folate receptor 4 as indicator, method for treating diseases using the detection method, pharmaceutical composition for immunostimulation, and method for treating diseases using the composition
Abstract (US7488474)
An object of the present invention is to provide a technique to distinguish between Treg cells and activated T cells in a live state.
Another object of the present invention is to provide a pharmaceutical composition for immunostimulation that can reduce the number of Treg cells in vivo and effectively express the immune response of activated T cells.
In the method of the present invention, Treg cells are detected from test cells containing (i) regulatory T cells and (ii) at least one type of cell selected from the group consisting of naive T cells and activated T cells, wherein expressions of folate receptor 4 on the surfaces of cells are measured and Treg cells are detected using the expressions as an indicator.
The present invention uses anti-folate receptor 4 antibody or folate receptor 4-binding fragment as an active ingredient contained in a pharmaceutical composition for immunostimulation.
Scope of claims [claim1]
1. A method for treating a malignant tumor or infectious disease comprising:
(a) collecting cells comprising T cells from a patient suffering from a malignant tumor or infectious disease;(b) inducing the T cells in the cells collected in (a) by subjecting the cells collected to an antigenic stimulus in vitro;(c) depleting from the resulting cells of (b), cells having the highest folate receptor 4 expression, so as to obtain cells comprising T cells from which regulatory T cells have been removed;
and(d) administering the resulting depleted cells obtained in (c) to the patient.
[claim2]
2. The method of claim 1, wherein step (c) is carried out by the method comprising:
(i) measuring expression of folate receptor 4 on the surface of the T cells;(ii) detecting regulatory T cells using the expression as an indicator;
and(iii) depleting regulatory T cells having the highest folate receptor 4 expression.
[claim3]
3. The method of claim 1, wherein the folate receptor 4 expression is measured using an anti-folate receptor 4 antibody or a folate receptor 4-binding fragment.
  • Inventor, and Inventor/Applicant
  • SAKAGUCHI SHIMON
  • HIROTA KEIJI
  • YAMAGUCHI TOMOYUKI
  • KYOTO UNIVERSITY
  • RIKEN
IPC(International Patent Classification)
U.S. Cl./(Sub)
  • C07K016/28
  • G01N033/569H2
  • K61K039/505
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