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ENZYME STORAGE SOLUTION AND REACTION SOLUTION FOR RECONSTRUCTED CELL-FREE TRANSLATION SYSTEM USING SAME

Foreign code F150008479
File No. E102P08WO
Posted date Oct 26, 2015
Country WIPO
International application number 2014JP071707
International publication number WO 2015025865
Date of international filing Aug 20, 2014
Date of international publication Feb 26, 2015
Priority data
  • P2013-171347 (Aug 21, 2013) JP
Title ENZYME STORAGE SOLUTION AND REACTION SOLUTION FOR RECONSTRUCTED CELL-FREE TRANSLATION SYSTEM USING SAME
Abstract Provided are: an enzyme storage solution for a reconstructed cell-free translation system in which the concentration of chloride salts is 10 mM or less and the glycerol concentration is 5% or less; and a reaction solution for a reconstructed cell-free translation system, which is able to markedly improve the efficiency of protein synthesis compared to the past using a reaction solution for a reconstructed cell-free translation system that comprises said storage solution.
Scope of claims (In Japanese)[請求項1]
塩化物塩の濃度が10mM以下であり、かつ、グリセロールの濃度が5%以下である、酵素の保存液。
[請求項2]
塩化物塩を含まない、請求項1に記載の保存液。
[請求項3]
塩化物塩が塩化カリウムおよび塩化マグネシウムの少なくともいずれかである、請求項2に記載の保存液。
[請求項4]
グリセロールを含まない、請求項1~3のいずれか1項に記載の保存液。
[請求項5]
グルタミン酸カリウム、酢酸カリウムまたはアスパラギン酸カリウムを含む、請求項1~4のいずれか1項に記載の保存液。
[請求項6]
グルタミン酸マグネシウム、酢酸マグネシウムまたはアスパラギン酸マグネシウムを含む、請求項1~5のいずれか1項に記載の保存液。
[請求項7]
再構成型無細胞翻訳システムに用いるための酵素の保存液である、請求項1~6のいずれか1項に記載の保存液。
[請求項8]
請求項1~7のいずれか1項に記載の保存液を含む、再構成型無細胞翻訳システム用反応液。
  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • Inventor
  • YOMO TETSUYA
  • MATSUURA TOMOAKI
  • KAZUTA YASUAKI
IPC(International Patent Classification)
Reference ( R and D project ) ERATO YOMO Dynamical Micro-scale Reaction Environment AREA
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