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MODIFIED Β-GLUCURONIDASE

外国特許コード F150008484
整理番号 E102P07WO
掲載日 2015年10月26日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2014JP069539
国際公開番号 WO 2015016124
国際出願日 平成26年7月24日(2014.7.24)
国際公開日 平成27年2月5日(2015.2.5)
優先権データ
  • 特願2013-156615 (2013.7.29) JP
発明の名称 (英語) MODIFIED Β-GLUCURONIDASE
発明の概要(英語) Provided is a modified β-glucuronidase in which a mutation has been introduced to the amino acid sequence of a β-glucuronidase monomer, and wherein the modified β-glucuronidase is mutated and novel and has methionine or serine at the 561st position from the N terminal side of the amino acid sequence, and due to being a modified β-glucuronidase, has high enzyme activity when compared to the wild form, and is easily detected.
従来技術、競合技術の概要(英語) BACKGROUND ART
Such as within a cell to express the gene of interest in for analyzing, quantifying the transcription of the gene product, is a need to identify. However, in the case of directly analyzing the transcripts, purified mRNA, analyzing the hybridization of the electrophoresis takes time and, further, the internalized by the cell needs to be distinguished from other mRNA is also a careful manipulation is required. As a useful technique in such a case, using a reporter gene encoding an enzyme, which can be analyzed to indirectly known.
Β - glucuronidase gene, a plant cell that does not have a particularly useful are hitherto used as a reporter gene. In the case of using β - glucuronidase gene as a reporter gene, the expression of the gene from the β - glucuronidase. Β - glucuronidase is, in the state of monomer activity not shown, tetramer only in its activity, its detection is enabled. As an example of the method of assembling a conventional, that are assembled to the plate perpendicular to one another by screwing a screw. By previous studies, the activity of the β - glucuronidase expression, depending on the size of cells and requires considerable time proved to be point (for example, t. Matsuura et al., "Kinetic Analysis of β-Galactosidase and β-Glucuronidase Tetramerization Coupled with Protein Translation", Journal of Biological Chemistry ., (2011) 286, 22028 reference (non-patent document 1). ).
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • YOMO, Tetsuya
  • SUNAMI, Takeshi
  • NISHIKAWA, Takehiro
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN TD TG
参考情報 (研究プロジェクト等) ERATO YOMO Dynamical Micro-scale Reaction Environment AREA
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