TOP > 外国特許検索 > METHOD FOR ANALYSIS FOR 3-HYDROXYPROLINE, METHOD FOR EXAMINING COLLAGEN, AND NOVEL Δ1-PYRROLINE-2-CARBOXYLIC ACID REDUCTASE FOR USE THEREIN

METHOD FOR ANALYSIS FOR 3-HYDROXYPROLINE, METHOD FOR EXAMINING COLLAGEN, AND NOVEL Δ1-PYRROLINE-2-CARBOXYLIC ACID REDUCTASE FOR USE THEREIN

外国特許コード F150008507
整理番号 S2013-0983-C0
掲載日 2015年11月5日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2014JP065019
国際公開番号 WO 2015033636
国際出願日 平成26年6月5日(2014.6.5)
国際公開日 平成27年3月12日(2015.3.12)
優先権データ
  • 特願2013-186647 (2013.9.9) JP
発明の名称 (英語) METHOD FOR ANALYSIS FOR 3-HYDROXYPROLINE, METHOD FOR EXAMINING COLLAGEN, AND NOVEL Δ1-PYRROLINE-2-CARBOXYLIC ACID REDUCTASE FOR USE THEREIN
発明の概要(英語) The purpose of the present invention is to provide a new analytical method which renders simple analysis for 3-hydroxyproline possible. A sample to be examined is analyzed for 3-hydroxyproline through the following steps (s1) to (s3): (s1) a dehydration step in which the 3-hydroxyproline contained in the sample to be examined is dehydrated into Δ1 -pyrroline-2-carboxylic acid by the action of a 3-hydroxyproline dehydratase; (s2) a reduction step in which the Δ1 -pyrroline-2-carboxylic acid obtained in step (s1) is hydrogenated by the action of a Δ1 -pyrroline-2-carboxylic acid reductase; and (s3) an analysis step in which the reduction reaction in step (s2) is analyzed.
従来技術、競合技術の概要(英語) BACKGROUND ART
In recent years, from the viewpoint of health and cosmetically, attracted attention and is collagen, a quantitative analysis and qualitative analysis of collagen, has been regarded as important.
As an analysis method of collagen in the sample, and measuring the content of hydroxyproline contained in the collagen by, a method of measuring indirectly generally collagen. Collagen, general protein, non-collagenous proteins that do not correspond to the amino acid is a basic element containing hydroxyproline. For this reason, can analyze the hydroxyproline in the sample, indirectly, of collagen in the sample analysis can be made. Then, measurement of hydroxyproline, a general-purpose analysis HPLC (patent document 1). As a specific method is, first, the pre-processing the sample, by the degradation of the collagen to liberate hydroxyproline. The pre-processed sample was subjected to HPLC, to calculate the amount of hydroxyproline from the peak area of hydroxyproline. Then, and the calculated value, and the amount of hydroxyproline per molecule collagen 1, the amount of collagen in the sample can be obtained.
When analyzing HPLC analysis hydroxyproline, hydroxyproline, liberated by the degradation of the collagen must be separated from the hydroxyproline L -. However, analyzed by HPLC, and the separation of hydroxyproline and hydroxyproline L -, difficult. In addition, hydroxyproline may be a primary amine 2, special developing solvent and the ring-opening is necessary. Further, HPLC analysis, and the analytical column and the required device expensive, a problem that time is required for the analysis.
On the other hand, among them collagen, collagen IV is, the concentration in the urine of cancer patients and reported to be elevated, which may be a marker for cancer. Then, the collagen IV, other types (for example, I-III) compared to, hydroxyproline is about 8-25 times from the contained 3 -, 3 - by analyzing hydroxyproline, collagen IV and indirectly a cancer marker can be analyzed. For this reason, a simple analysis of hydroxyproline 3 - is able to be demanded.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • NATIONAL UNIVERSITY CORPORATION EHIME UNIVERSITY
  • 発明者(英語)
  • WATANABE Seiya
  • TANIMOTO Yoshiaki
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN TD TG
四国TLOは四国内20大学・高専のシーズをご紹介しております。シーズの詳細内容につきましては下記までお問い合わせください。
お問い合わせをお待ちしております。

PAGE TOP

close
close
close
close
close
close