Nucleic acid complex and nucleic acid-polysaccharide complex
| Foreign code | F150008513 |
|---|---|
| File No. | AF11-09TW |
| Posted date | Nov 19, 2015 |
| Country | Taiwan |
| Application number | 102121892 |
| Gazette No. | 201400496 |
| Gazette No. | I606058 |
| Date of filing | Jun 20, 2013 |
| Gazette Date | Jan 1, 2014 |
| Gazette Date | Nov 21, 2017 |
| Priority data |
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| Title | Nucleic acid complex and nucleic acid-polysaccharide complex |
| Abstract |
(TWI606058) As a means for solving the problem of providing a nucleic acid complex that rarely undergoes the decomposition at a DNA-RNA joining site therein even in vivo, a nucleic acid complex is provided, which is produced by linking 3'-position of a 3'-terminal deoxyribonucleotide residue in single-stranded DNA to 5'-position of a 5'-terminal ribonucleotide residue in one ribonucleotide strand in double-stranded RNA, wherein a hydroxyl group located at 2'-position of the 5'-terminal nucleotide in the ribonucleotide strand that is bound to the single-stranded DNA is substituted by an alkoxy group or a halogen group and/or a phosphoric diester group between 3'-position of the first ribonucleotide that is bound to the single-stranded DNA and 5'-position of a ribonucleotide adjacent to the first ribonucleotide is substituted by any one selected from a phosphorothioate group, a dithiophosphoric diester group and a trithiophosphoric diester group. |
| Scope of claims |
[claim1] 1. Kinds of nucleic acid complexes, it is by 3rd single stranded DNA ' 3rd side deacidizing of terminal ribose nucleotide residue ' position and with nucleic acid complex that 5th of ribose double strand RNA nucleotide chain ' 5th side of terminal ribonucleic acid residue ' position key ties to form, and ties 5th this ribose nucleotide chain with this single stranded DNA key ' in side terminal nucleotide 2 ' hydroxy of fundamental series position after alkoxyl or halogen atom substitution. [claim2] 2. Like nucleic acid of complex request item of 1 station, nucleotide of number this single stranded DNA is over 10. [claim3] 3. Like nucleic acid of complex request item of 1 station, this single stranded DNA is the gathering deacidizing adenine. [claim4] 4. Like nucleic acid of complex request item of 1 station, at least of a sub-system this single stranded di-phosphate ester of DNA bases after sulfo- phosphate base and dithio- di-phosphate ester base and thio di-phosphate ester bases any of substitution. [claim5] 5. Like nucleic acid of complexes request item of 4 station, in this single stranded DNA di-phosphate ester of fundamental series at least over 50% after sulfo- phosphate base and dithio- di-phosphate ester base and thio di-phosphate ester bases any of substitution. [claim6] 6. Like nucleic acid of complex request item of 1 station, this double strand RNA is siRNA. [claim7] 7. Like nucleic acid of complexes request item of 6 station, this siRNA is 21mer or 27mer. [claim8] 8. Like nucleic acid of complexes request item of 6 station, goal of base this siRNA, because expressed in Dectin-1 the cell the gene of expression. [claim9] 9. 1 kinds of nucleic acid complexes, it is by the nucleic acid complex that 3rd single stranded DNA ' 3rd side deacidizing of terminal ribose nucleotide residue ' 5th of ribose position and double strand RNA nucleotide chain ' 5th side ribose of terminal nucleotide residue ' position key ties to become, and ties ribose nucleotide with this single stranded DNA key 3rd at first ' position and ' the di-phosphate ester fundamental series between positions with ribose of its adjacency nucleotide 5th after the sulfo- phosphate base and dithio- di-phosphate ester base and thio di-phosphate ester bases any of the substitution. [claim10] 10. Like nucleic acid of complexes request item of 9 station, nucleotide of number this single stranded DNA is over 10. [claim11] 11. Like nucleic acid of complexes request item of 9 station, this single stranded DNA is the gathering deacidizing adenine. [claim12] 12. Like nucleic acid of complexes request item of 9 station, at least of a sub-system this single stranded di-phosphate ester of DNA bases after sulfo- phosphate base and dithio- di-phosphate ester base and thio di-phosphate ester bases any of substitution. [claim13] 13. Like nucleic acid of complexes request item of 12 station, in this single stranded DNA di-phosphate ester of fundamental series at least over 50% after sulfo- phosphate base and dithio- di-phosphate ester base and thio di-phosphate ester bases any of substitution. [claim14] 14. Like nucleic acid of complexes request item of 9 station, this double strand RNA is siRNA. [claim15] 15. Like nucleic acid of complexes request item of 14 station, this siRNA is 21mer or 27mer. [claim16] 16. Like nucleic acid of complexes request item of 14 station, goal of base this siRNA, because expressed in Dectin-1 the cell the gene of expression. [claim17] 17. 1 kinds of nucleic acid polysaccharide complexes, it is like the request item states the single stranded of DNA spot nucleic acid complex to have 1 to the 16 any item of institute with 2 members by 1 member β-1,3 - the polysaccharide of glucosan skeleton forms the nucleic acid of polysaccharide complex tertiary spiral structure. [claim18] 18. Like nucleic acid of polysaccharide complexes request item of 17 station, should have β-1,3 - the polysaccharide of glucosan skeleton for crack pleat fungus polysaccharide. [claim19] 19. 1 kinds of medicine fabrics, contain like the nucleic acid of polysaccharide complexes request item of 17 station. |
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| Reference ( R and D project ) | CREST Development of the Foundation for Nano-Interface Technology AREA |
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Contact Information for " Nucleic acid complex and nucleic acid-polysaccharide complex "
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- URL: http://www.jst.go.jp/chizai/
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