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Nucleic acid complex and nucleic acid-polysaccharide complex

Foreign code F150008513
File No. AF11-09TW
Posted date Nov 19, 2015
Country Taiwan
Application number 102121892
Gazette No. 201400496
Gazette No. I606058
Date of filing Jun 20, 2013
Gazette Date Jan 1, 2014
Gazette Date Nov 21, 2017
Priority data
  • P2012-139250 (Jun 20, 2012) JP
Title Nucleic acid complex and nucleic acid-polysaccharide complex
Abstract (TWI606058)
As a means for solving the problem of providing a nucleic acid complex that rarely undergoes the decomposition at a DNA-RNA joining site therein even in vivo, a nucleic acid complex is provided, which is produced by linking 3'-position of a 3'-terminal deoxyribonucleotide residue in single-stranded DNA to 5'-position of a 5'-terminal ribonucleotide residue in one ribonucleotide strand in double-stranded RNA, wherein a hydroxyl group located at 2'-position of the 5'-terminal nucleotide in the ribonucleotide strand that is bound to the single-stranded DNA is substituted by an alkoxy group or a halogen group and/or a phosphoric diester group between 3'-position of the first ribonucleotide that is bound to the single-stranded DNA and 5'-position of a ribonucleotide adjacent to the first ribonucleotide is substituted by any one selected from a phosphorothioate group, a dithiophosphoric diester group and a trithiophosphoric diester group.
Scope of claims [claim1]
1. Kinds of nucleic acid complexes, it is by 3rd single stranded DNA ' 3rd side deacidizing of terminal ribose nucleotide residue ' position and with nucleic acid complex that 5th of ribose double strand RNA nucleotide chain ' 5th side of terminal ribonucleic acid residue ' position key ties to form, and ties 5th this ribose nucleotide chain with this single stranded DNA key ' in side terminal nucleotide 2 ' hydroxy of fundamental series position after alkoxyl or halogen atom substitution.
[claim2]
2. Like nucleic acid of complex request item of 1 station, nucleotide of number this single stranded DNA is over 10.
[claim3]
3. Like nucleic acid of complex request item of 1 station, this single stranded DNA is the gathering deacidizing adenine.
[claim4]
4. Like nucleic acid of complex request item of 1 station, at least of a sub-system this single stranded di-phosphate ester of DNA bases after sulfo- phosphate base and dithio- di-phosphate ester base and thio di-phosphate ester bases any of substitution.
[claim5]
5. Like nucleic acid of complexes request item of 4 station, in this single stranded DNA di-phosphate ester of fundamental series at least over 50% after sulfo- phosphate base and dithio- di-phosphate ester base and thio di-phosphate ester bases any of substitution.
[claim6]
6. Like nucleic acid of complex request item of 1 station, this double strand RNA is siRNA.
[claim7]
7. Like nucleic acid of complexes request item of 6 station, this siRNA is 21mer or 27mer.
[claim8]
8. Like nucleic acid of complexes request item of 6 station, goal of base this siRNA, because expressed in Dectin-1 the cell the gene of expression.
[claim9]
9. 1 kinds of nucleic acid complexes, it is by the nucleic acid complex that 3rd single stranded DNA ' 3rd side deacidizing of terminal ribose nucleotide residue ' 5th of ribose position and double strand RNA nucleotide chain ' 5th side ribose of terminal nucleotide residue ' position key ties to become, and ties ribose nucleotide with this single stranded DNA key 3rd at first ' position and ' the di-phosphate ester fundamental series between positions with ribose of its adjacency nucleotide 5th after the sulfo- phosphate base and dithio- di-phosphate ester base and thio di-phosphate ester bases any of the substitution.
[claim10]
10. Like nucleic acid of complexes request item of 9 station, nucleotide of number this single stranded DNA is over 10.
[claim11]
11. Like nucleic acid of complexes request item of 9 station, this single stranded DNA is the gathering deacidizing adenine.
[claim12]
12. Like nucleic acid of complexes request item of 9 station, at least of a sub-system this single stranded di-phosphate ester of DNA bases after sulfo- phosphate base and dithio- di-phosphate ester base and thio di-phosphate ester bases any of substitution.
[claim13]
13. Like nucleic acid of complexes request item of 12 station, in this single stranded DNA di-phosphate ester of fundamental series at least over 50% after sulfo- phosphate base and dithio- di-phosphate ester base and thio di-phosphate ester bases any of substitution.
[claim14]
14. Like nucleic acid of complexes request item of 9 station, this double strand RNA is siRNA.
[claim15]
15. Like nucleic acid of complexes request item of 14 station, this siRNA is 21mer or 27mer.
[claim16]
16. Like nucleic acid of complexes request item of 14 station, goal of base this siRNA, because expressed in Dectin-1 the cell the gene of expression.
[claim17]
17. 1 kinds of nucleic acid polysaccharide complexes, it is like the request item states the single stranded of DNA spot nucleic acid complex to have 1 to the 16 any item of institute with 2 members by 1 member β-1,3 - the polysaccharide of glucosan skeleton forms the nucleic acid of polysaccharide complex tertiary spiral structure.
[claim18]
18. Like nucleic acid of polysaccharide complexes request item of 17 station, should have β-1,3 - the polysaccharide of glucosan skeleton for crack pleat fungus polysaccharide.
[claim19]
19. 1 kinds of medicine fabrics, contain like the nucleic acid of polysaccharide complexes request item of 17 station.
  • Applicant
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • Inventor
  • SAKURAI KAZUO
  • MOCHIZUKI SHIN-ICHI
  • TANAKA MOTOKO
  • HIGUCHI SADAHARU
IPC(International Patent Classification)
Reference ( R and D project ) CREST Development of the Foundation for Nano-Interface Technology AREA
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