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HIGH-EXPRESSION PROMOTER

Foreign code F160008706
File No. (S2014-1218-N0)
Posted date Mar 30, 2016
Country WIPO
International application number 2015JP003793
International publication number WO 2016021149
Date of international filing Jul 29, 2015
Date of international publication Feb 11, 2016
Priority data
  • P2014-158646 (Aug 4, 2014) JP
Title HIGH-EXPRESSION PROMOTER
Abstract The present invention addresses the problem of providing a promoter that yields higher expression than the TDH3 promoter, the GAL10 promoter, or the like. A promoter that is for causing a target gene to be more highly expressed in yeast. The promoter is prepared so as to comprise, in order, a yeast TDH3 or GAL10 promoter sequence and an intron sequence for a yeast ribosomal protein gene, the intron sequence for the yeast ribosomal protein gene being any of sequences (a)-(c): (a) a base sequence represented by SEQ ID NO:1-4; (b) a sequence that is a base sequence represented by SEQ ID NO:1-4 wherein one or several bases have been deleted, added, inserted, or substituted; (c) a sequence that has 90% or more identity with a base sequence represented by SEQ ID NO:1-4.
Scope of claims (In Japanese)[請求項1]
酵母において目的遺伝子を高発現させるためのプロモーターであって、酵母のTDH3又はGAL10遺伝子のプロモーター配列と、酵母のリボソーマルタンパク質遺伝子のイントロン配列とを順次備えており、前記酵母のリボソーマルタンパク質遺伝子のイントロン配列が、以下に示す(a)~(c)のいずれかの配列であることを特徴とするプロモーター。
(a)配列番号1~4に示す塩基配列;
(b)配列番号1~4に示す塩基配列において、1又は数個の塩基が欠失、置換、付加、若しくは挿入された配列;
(c)配列番号1~4に示す塩基配列と90%以上の同一性を有する配列;
[請求項2]
酵母が、サッカロマイセス・セレビシエであることを特徴とする請求項1記載のプロモーター。
[請求項3]
請求項1又は2記載のプロモーターを含む組換えベクター。
[請求項4]
請求項3記載の組換えベクターが導入された酵母の形質転換体。
  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • YAMAGUCHI UNIVERSITY
  • Inventor
  • HOSHIDA HISASHI
  • AKADA RINJI
IPC(International Patent Classification)
Specified countries National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG

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