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ALGAE AND METHOD FOR PRODUCING SAME, AND METHOD FOR PRODUCING BIOMASS USING SAID ALGAE

外国特許コード F160008718
整理番号 (AF21P004)
掲載日 2016年4月14日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2015JP002634
国際公開番号 WO 2015182110
国際出願日 平成27年5月26日(2015.5.26)
国際公開日 平成27年12月3日(2015.12.3)
優先権データ
  • 特願2014-111577 (2014.5.29) JP
発明の名称 (英語) ALGAE AND METHOD FOR PRODUCING SAME, AND METHOD FOR PRODUCING BIOMASS USING SAID ALGAE
発明の概要(英語) The productivity of biomass within the cells of algae can be improved by suppressing the expression of ATG8 in algae by (i) overexpression of MEX1 and/or (ii) silencing using miRNA, thereby providing algae having improved productivity of photosynthetic products, a method for producing said algae, a method for producing a biomass using said algae, and starch obtained by said algae.
従来技術、競合技術の概要(英語) BACKGROUND ART
As fuel alternatives to fossil fuels, biomass-derived fuel, so-called bio fuel (for example, bioethanol, biodiesel and the like) is expected.
A raw material of biomass (for example, starch) or sugars such as oil or fat biomass, plant is produced by performing photosynthesis. For this reason, and actively photosynthesis, sugars oils or fats such as plants that have the ability to accumulate inside the cell, can be utilized as the biomass producing means. The current, in order to produce biomass, mainly, corn or soybean used. Corn or soybean, are also used as a food or feed. For this reason, doubled significantly caused by the production of biomass, the price of the food and feed has been regarded as a problem.
Therefore, as an alternative to corn or soybean biomass production, production by the algae biomass has attracted attention (for example, see Patent Document 1 and Patent Document 2). Biomass production by the algae is, not in conflict with the feed or food, can be mass proliferation, and the like advantages.
For example, for a kind of algae Chlamydomonas, cell wall deficient variants or cell wall becomes thinner variants (cw15, cw92 or the like) are known. These variants can, when introduced into cells from the outside DNA of convenient nature, has been widely used in gene transfer experiments. The fragile nature of the cells, facilitates recovery of cellular contents in the sense that they improve the productivity of the biomass, using these mutants have been reported for biomass production. For example, Patent Document 3 is, in the cell wall deficient Chlamydomonas mutants of technology for the production of fats and oils described. In addition, the non-patent document 1 is, in addition to the cell wall mutation (cw15), starch synthesis in Chlamydomonas vacancy, the oil droplets made from fats and oils can be discharged to the outside of the cells have been reported. Non-patent document 2 is, in the cell wall of the variant (cw15) Chlamydomonas, starch synthesis to disrupt a gene by increasing the productivity of the fat have been reported. In addition, as a report regarding mutations Chlamydomonas cell wall, non-patent document 3 is known.
In addition, as a material which is a kind of algae Chlorella, produced outside of the cell to release the starch, followed by ethanol fermentation techniques have been reported (patent document 4). Further, the chloroplast of algae in the body by increasing the glutathione concentration, starch so as to enhance the production capabilities have been reported (patent document 5).
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • OGAWA, Ken'ichi
  • NISHIKAWA, Masanobu
  • KIYOKAWA, Kazuya
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
参考情報 (研究プロジェクト等) CREST Creation of Innovative Technologies to Control Carbon Dioxide Emissions AREA
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