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ALGAE AND METHOD FOR PRODUCING SAME, AND METHOD FOR PRODUCING BIOMASS USING SAID ALGAE

Foreign code F160008718
File No. (AF21P004)
Posted date Apr 14, 2016
Country WIPO
International application number 2015JP002634
International publication number WO 2015182110
Date of international filing May 26, 2015
Date of international publication Dec 3, 2015
Priority data
  • P2014-111577 (May 29, 2014) JP
Title ALGAE AND METHOD FOR PRODUCING SAME, AND METHOD FOR PRODUCING BIOMASS USING SAID ALGAE
Abstract The productivity of biomass within the cells of algae can be improved by suppressing the expression of ATG8 in algae by (i) overexpression of MEX1 and/or (ii) silencing using miRNA, thereby providing algae having improved productivity of photosynthetic products, a method for producing said algae, a method for producing a biomass using said algae, and starch obtained by said algae.
Outline of related art and contending technology BACKGROUND ART
As fuel alternatives to fossil fuels, biomass-derived fuel, so-called bio fuel (for example, bioethanol, biodiesel and the like) is expected.
A raw material of biomass (for example, starch) or sugars such as oil or fat biomass, plant is produced by performing photosynthesis. For this reason, and actively photosynthesis, sugars oils or fats such as plants that have the ability to accumulate inside the cell, can be utilized as the biomass producing means. The current, in order to produce biomass, mainly, corn or soybean used. Corn or soybean, are also used as a food or feed. For this reason, doubled significantly caused by the production of biomass, the price of the food and feed has been regarded as a problem.
Therefore, as an alternative to corn or soybean biomass production, production by the algae biomass has attracted attention (for example, see Patent Document 1 and Patent Document 2). Biomass production by the algae is, not in conflict with the feed or food, can be mass proliferation, and the like advantages.
For example, for a kind of algae Chlamydomonas, cell wall deficient variants or cell wall becomes thinner variants (cw15, cw92 or the like) are known. These variants can, when introduced into cells from the outside DNA of convenient nature, has been widely used in gene transfer experiments. The fragile nature of the cells, facilitates recovery of cellular contents in the sense that they improve the productivity of the biomass, using these mutants have been reported for biomass production. For example, Patent Document 3 is, in the cell wall deficient Chlamydomonas mutants of technology for the production of fats and oils described. In addition, the non-patent document 1 is, in addition to the cell wall mutation (cw15), starch synthesis in Chlamydomonas vacancy, the oil droplets made from fats and oils can be discharged to the outside of the cells have been reported. Non-patent document 2 is, in the cell wall of the variant (cw15) Chlamydomonas, starch synthesis to disrupt a gene by increasing the productivity of the fat have been reported. In addition, as a report regarding mutations Chlamydomonas cell wall, non-patent document 3 is known.
In addition, as a material which is a kind of algae Chlorella, produced outside of the cell to release the starch, followed by ethanol fermentation techniques have been reported (patent document 4). Further, the chloroplast of algae in the body by increasing the glutathione concentration, starch so as to enhance the production capabilities have been reported (patent document 5).
Scope of claims (In Japanese)[請求項1]
ATG8の発現が基準株に比して抑制された藻類を用いる、バイオマスの製造方法。
[請求項2]
前記藻類に対して光を照射する光照射工程を含む、請求項1に記載のバイオマスの製造方法。
[請求項3]
葉緑体内のグルタチオン濃度が基準株に比して増加した藻類を用いる、請求項1または2に記載のバイオマスの製造方法。
[請求項4]
前記光照射工程は、実質的に窒素飢餓ではない条件において行われる、請求項3に記載のバイオマスの製造方法。
[請求項5]
藻類の細胞を破砕する細胞破砕工程を含まない、請求項4に記載のバイオマスの製造方法。
[請求項6]
ATG8の発現が基準株に比して抑制された藻類。
[請求項7]
MEX1が過剰発現されている、請求項6に記載の藻類。
[請求項8]
MEX1をコードする外因性ポリヌクレオチドが導入されている、請求項7に記載の藻類。
[請求項9]
前記外因性ポリヌクレオチドが、以下の(a)~(c)からなる群より選択される一以上である、請求項8に記載の藻類:
(a)配列番号1または3に示されるアミノ酸配列からなるポリペプチドをコードするポリヌクレオチド;
(b)配列番号1または3に示されるアミノ酸配列において、1または数個のアミノ酸が欠失、置換もしくは付加されたアミノ酸配列からなり、かつMEX1の機能を保持するポリペプチドをコードするポリヌクレオチド;
(c)前記(a)または(b)のポリヌクレオチドのうちいずれかのポリヌクレオチドに相補的な塩基配列からなるポリヌクレオチドとストリンジェントな条件下でハイブリダイズし、かつMEX1の機能を保持するポリペプチドをコードするポリヌクレオチド。
[請求項10]
ATG8遺伝子がサイレンシングされている、請求項6に記載の藻類。
[請求項11]
配列番号5に示される塩基配列を有するmiRNAが導入されている、請求項10に記載の藻類。
[請求項12]
葉緑体内のグルタチオン濃度が基準株に比して増加している、請求項6~11のいずれか1項に記載の藻類。
[請求項13]
ATG8の発現を抑制するATG8発現抑制工程を含む、改変藻類の製造方法。
[請求項14]
葉緑体内のグルタチオン濃度を増加させるグルタチオン濃度増加工程を含む、請求項13に記載の改変藻類の製造方法。
[請求項15]
ATG8の発現が基準株に比して抑制された藻類を用いて取得されるデンプン。
[請求項16]
葉緑体内のグルタチオン濃度が基準株に比して増加している藻類を用いて取得される、請求項15に記載のデンプン。
  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • Inventor
  • OGAWA, Ken'ichi
  • NISHIKAWA, Masanobu
  • KIYOKAWA, Kazuya
IPC(International Patent Classification)
Specified countries National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
Reference ( R and D project ) CREST Creation of Innovative Technologies to Control Carbon Dioxide Emissions AREA
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