Top > Search of International Patents > METHOD OF PREPARING FUNCTIONALLY ACTIVATED CELL SHEET THROUGH LOW OXYGEN TREATMENT

METHOD OF PREPARING FUNCTIONALLY ACTIVATED CELL SHEET THROUGH LOW OXYGEN TREATMENT

Foreign code F160008785
File No. (S2014-1298-N0)
Posted date Aug 4, 2016
Country WIPO
International application number 2015JP076207
International publication number WO 2016043201
Date of international filing Sep 16, 2015
Date of international publication Mar 24, 2016
Priority data
  • P2014-188322 (Sep 16, 2014) JP
Title METHOD OF PREPARING FUNCTIONALLY ACTIVATED CELL SHEET THROUGH LOW OXYGEN TREATMENT
Abstract The present invention provides a method of preparing a cell sheet which is more usefully used in the treatment of damage in living tissue. In particular, the present invention provides a method for preparing a cell sheet which achieves a sufficient functional improvement of an infarcted myocardium by transplanting the cell sheet for an infarcted myocardium in myocardial infarction. More specifically, the present invention relates to a method of preparing a cell sheet, the method comprising: a step for culturing cells on a culture substrate to form a cell sheet derived from the cells; a step for culturing the cell sheet under a low oxygen condition at a predetermined temperature for a predetermined period of time; and a step for detaching the cell sheet from the culture substrate after cultured under said condition.
Scope of claims [claim1]
1.  Method for manufacturing a cell sheet comprising the following of (a) ~ (c) step. (A) culturing the cells on a culture substrate, the step of forming the cell sheet from the cells, (B) the cell sheet at a predetermined temperature and low oxygen conditions, the step of a predetermined period of culture, (C) After the culture at the conditions, the process of peeling the cell sheet from the culture substrate
[2]
[claim2]
2.  The method of claim 1 wherein the cell, which is a Cardiosphere derived cells.
[3]
[claim3]
3.  The hypoxic conditions, method according to claim 1 or 2, wherein the oxygen concentration of 0% to 8%.
[4]
[claim4]
4.  A method according to any one of claims 1 to 3 wherein the predetermined temperature, characterized in that it is a 30 ° C ~ 36 ° C.
[5]
[claim5]
5.  The culture period is, the method according to any one of claims 1 to 4, characterized in that the 12 to 72 hours.
[6]
[claim6]
6.  Cell sheet produced by the method according to any one of claims 1 to 5.
  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • YAMAGUCHI UNIVERSITY
  • Inventor
  • HAMANO KIMIKAZU
  • HOSOYAMA TOHRU
  • UENO KOJI
IPC(International Patent Classification)
Specified countries National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG

PAGE TOP

close
close
close
close
close
close