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NOVEL DIATOM TRANSFORMATION VECTOR AND NOVEL PROMOTER SEQUENCE INCLUDING SAME 新技術説明会

外国特許コード F160008808
整理番号 (S2014-1454-N0)
掲載日 2016年8月4日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2015JP075372
国際公開番号 WO 2016039300
国際出願日 平成27年9月7日(2015.9.7)
国際公開日 平成28年3月17日(2016.3.17)
優先権データ
  • 特願2014-182607 (2014.9.8) JP
発明の名称 (英語) NOVEL DIATOM TRANSFORMATION VECTOR AND NOVEL PROMOTER SEQUENCE INCLUDING SAME 新技術説明会
発明の概要(英語) The invention provides a novel promoter for transforming algae (e.g., diatoms) having a base sequence of SEQ ID NOS: 1-10 or a base sequence having 80% or higher homology with base this sequence, a novel transformation vector including this promoter, and a method for transforming algae using this vector.
従来技術、競合技術の概要(英語) BACKGROUND ART
1 Is one of the marine diatom algae, belonging to the group of so called yellow plant referred to as a single cell eukaryote. The world diatoms CO2 degree also a fixed amount of 20-40% is estimated to be responsible for the, primary producer organism groups to the earth environment is very important. Diatoms is, EPA (eicosapentaenoic acid) such as typified by, such as fats and oils useful for the human organism is also synthesized. In general, to be used in the industrial organism, technologies are used in the transformation, the transformation efficiency of algae are known to be. In order to improve transformation efficiency, although a variety of research, transformation methods are still limiting. In particular, one of the first center marine diatoms diatomaceous protrusion will be 1, and a method of introducing the gene is its transformation efficiency is low. ThalassiosiraPseudonanaTpfcp/nat Chaetoceros sp. derived from a promoter containing plasmids for transformation, the transformation efficiency 1.5-6 transformants/108 cells very low, since the expression of the transgene has not been detected (non-patent document 1).
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • UNIVERSITY OF HYOGO
  • 発明者(英語)
  • IFUKU, Kentaro
  • KASHINO, Yasuhiro
  • FUKUZAWA, Hideya
  • KAJIKAWA, Masataka
  • OGAWA, Jun
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG

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