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NOVEL DIATOM TRANSFORMATION VECTOR AND NOVEL PROMOTER SEQUENCE INCLUDING SAME meetings

Foreign code F160008808
File No. (S2014-1454-N0)
Posted date Aug 4, 2016
Country WIPO
International application number 2015JP075372
International publication number WO 2016039300
Date of international filing Sep 7, 2015
Date of international publication Mar 17, 2016
Priority data
  • P2014-182607 (Sep 8, 2014) JP
Title NOVEL DIATOM TRANSFORMATION VECTOR AND NOVEL PROMOTER SEQUENCE INCLUDING SAME meetings
Abstract The invention provides a novel promoter for transforming algae (e.g., diatoms) having a base sequence of SEQ ID NOS: 1-10 or a base sequence having 80% or higher homology with base this sequence, a novel transformation vector including this promoter, and a method for transforming algae using this vector.
Outline of related art and contending technology BACKGROUND ART
1 Is one of the marine diatom algae, belonging to the group of so called yellow plant referred to as a single cell eukaryote. The world diatoms CO2 degree also a fixed amount of 20-40% is estimated to be responsible for the, primary producer organism groups to the earth environment is very important. Diatoms is, EPA (eicosapentaenoic acid) such as typified by, such as fats and oils useful for the human organism is also synthesized. In general, to be used in the industrial organism, technologies are used in the transformation, the transformation efficiency of algae are known to be. In order to improve transformation efficiency, although a variety of research, transformation methods are still limiting. In particular, one of the first center marine diatoms diatomaceous protrusion will be 1, and a method of introducing the gene is its transformation efficiency is low. ThalassiosiraPseudonanaTpfcp/nat Chaetoceros sp. derived from a promoter containing plasmids for transformation, the transformation efficiency 1.5-6 transformants/108 cells very low, since the expression of the transgene has not been detected (non-patent document 1).
Scope of claims (In Japanese)[請求項1]
(1)配列番号1~10いずれかの塩基配列からなるポリヌクレオチド;
(2)配列番号1~10いずれかの塩基配列に対して80%以上の相同性を有し、かつ藻類に目的遺伝子の発現をもたらすプロモーターとして機能するポリヌクレオチド;または
(3)配列番号1~10いずれかの塩基配列に対して1個または数個の塩基が付加、欠失および/または置換された塩基配列を有し、かつ藻類に目的遺伝子の発現をもたらすプロモーターとして機能するポリヌクレオチド。
[請求項2]
請求項1に記載のポリヌクレオチドを含むベクター。
[請求項3]
請求項1に記載のポリヌクレオチドおよびターミネーター配列を含む第1発現カセット;および
請求項1に記載のポリヌクレオチド、薬剤耐性遺伝子、およびターミネーター配列を含む第2発現カセット;
を含む請求項2に記載のベクター。
[請求項4]
請求項2または3のベクターを宿主藻類細胞へ導入することを特徴とする、藻類の形質転換方法。
[請求項5]
請求項1に記載のポリヌクレオチドおよび脂肪酸ヒドロキシラーゼ遺伝子を含むベクターを宿主藻類細胞へ導入して形質転換体を得、当該形質転換体を培養することによってリシノール酸を得ることを特徴とする、リシノール酸の製造方法。
  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • UNIVERSITY OF HYOGO
  • Inventor
  • IFUKU, Kentaro
  • KASHINO, Yasuhiro
  • FUKUZAWA, Hideya
  • KAJIKAWA, Masataka
  • OGAWA, Jun
IPC(International Patent Classification)
Specified countries National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG

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