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TARGET ANALYSIS CHIP AND TARGET ANALYSIS METHOD

外国特許コード F160008847
整理番号 (S2015-0396-N0)
掲載日 2016年9月23日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2016JP051925
国際公開番号 WO 2016117700
国際出願日 平成28年1月22日(2016.1.22)
国際公開日 平成28年7月28日(2016.7.28)
優先権データ
  • 特願2015-010639 (2015.1.22) JP
発明の名称 (英語) TARGET ANALYSIS CHIP AND TARGET ANALYSIS METHOD
発明の概要(英語) The present invention provides a novel target analysis chip and analysis method for directly detecting a target including micro RNAs without performing PCR. The target analysis chip according to the present invention includes a substrate. The substrate includes a reaction part where a sample containing a target is reacted with a reagent, a detection part that is an area where a label is to be detected, and a flow channel that connects the reaction part to the detection part. The reagent contains a carrier, and the carrier has an immobilized label probe to be bound to the target, and forms a bound body with the target. The flow channel includes a movement controlling means for controlling the movement of the carrier from the reaction part to the detection part. The movement controlling means includes a hydrophobic internal wall in the flow channel. When a centrifugal force (C2) larger than a resistance force (R) caused by the hydrophobicity of the flow channel is applied, the movement controlling means enables the bound body to move to the detection part through the flow channel.
従来技術、競合技術の概要(英語) BACKGROUND ART
In recent years, such as blood micro RNA RNA association with disease attracted attention and is, the detection of RNA, such as early detection of disease for medical use have been tried.
Of the micro RNA quantitation methods include an, for example, the target micro RNA specifically amplified by the polymerase (PCR) methods for is reported (patent document 1). However, which requires PCR, an apparatus and controlled temperature, and the like are necessary DNA reverse transcription, the operation becomes troublesome. In addition, in the methods, from a small amount of contained in the sample RNA, DNA obtained by reverse transcription, can be further amplified by the PCR. However, such a reverse transcription PCR is, in the sample or of the indirect detection of RNA, is not in a direct detection, in the case of quantitative analysis, a problem that the accuracy is thereby insufficient.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • ARKRAY, INC.
  • NATIONAL UNIVERSITY CORPORATION KYOTO INSTITUTE OF TECHNOLOGY
  • 発明者(英語)
  • MURAKAMI Akira
  • KOBORI Akio
  • YAMAYOSHI Asako
  • NODA Yuichiro
  • KONDO Masayuki
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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