TOP > 外国特許検索 > METHOD FOR SUPPRESSING PROTEIN ADSORPTION

METHOD FOR SUPPRESSING PROTEIN ADSORPTION

外国特許コード F160008862
整理番号 (S2015-0482-N0)
掲載日 2016年10月5日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2016JP054677
国際公開番号 WO 2016133152
国際出願日 平成28年2月18日(2016.2.18)
国際公開日 平成28年8月25日(2016.8.25)
優先権データ
  • 特願2015-031155 (2015.2.19) JP
発明の名称 (英語) METHOD FOR SUPPRESSING PROTEIN ADSORPTION
発明の概要(英語) The present invention addresses the problem of providing a novel method for suppressing the nonspecific adsorption of proteins. Knowledge enabling the abovementioned problem to be solved was obtained, leading to the completion of the present invention, by treating a solid phase in a solid-phase treatment solution having a phosphorylcholine group-containing polymer as the main component, treating a sample in a sample treatment solution having a polymer comprising a phosphorylcholine group, a hydroxyl group and a hydrophobic group as the main component, and then adding the treated sample to the treated solid phase.
従来技術、競合技術の概要(英語) BACKGROUND ART
The clinical test, in the field of diagnostic agents, immune reactions in a measuring method is widely used. In particular, has been required and high sensitivity inspection, clinical laboratory, improving the sensitivity of the diagnostic agent has been a great challenge. For attaining a high sensitivity, the detection method is a method using a peroxidase or alkaline phosphatase such as from the enzyme reaction, a method of using fluorescence or chemiluminescence is being switched to. Fluorescence or chemiluminescence detection method can be, in theory the inspection target material 1 can identify the presence of the molecule is said to be, in practice the target sensitivity is impossible.
Immune response when measured using one of the factors which affects the detection sensitivity, measuring the subject antibody, employed in the labeled antigen or immune reaction vessel and the solid phase surface and a non-specific adsorption. In addition, as the sample serum, plasma, cell extract and urine in the presence of a plurality of types of biomolecules such as in the case of using a material, such as various protein unspecified number of the coexisting materials in the reaction vessel and a solid phase surface to be an unspecific caused by the adsorption of the occurrence of noise, a factor for hindering high sensitivity.
In order to prevent nonspecific adsorption of these, in the prior art does not participate in the immune response of bovine serum albumin, casein, gelatin protein from one organism immune reaction vessel and on a solid phase surface by adsorbing a method of suppressing the nonspecific adsorption of the protein has been used. However, such as bovine serum albumin in the case of using the protein of the organism, which is represented by BSE problem of biofouling, the difference between lots, such as storage temperature and expiration date from a limitation, can solve the problem of the chemical composition as a main component in the development of protein nonspecific adsorption inhibitor has been desired.
The chemical composition as a main component as the nonspecific adsorption inhibitor is a protein, disclosed or less. Patent Document 1 is, 'the blocking agent comprises polyvinyl alcohol' has been disclosed. Patent Document 2 is, 'protein adsorption preventing agent containing a methacryloyloxyethylphosphorylcholine 2 -' has been disclosed. These methods, the protein to the solid phase surface chemical composition as the nonspecific adsorption inhibitor to effect only by the physical adsorption are expressed. These techniques may be, to some extent, to nonspecific adsorption of the solid phase surface can be avoided but, is not sufficient yet. Further, the sample treatment liquid of the present invention is not disclosed or suggested.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • NATIONAL UNIVERSITY CORPORATION KYOTO INSTITUTE OF TECHNOLOGY
  • NOF CORPORATION
  • 発明者(英語)
  • KUMADA YOICHI
  • KAWAGUCHI YASUTAKA
  • NEMOTO ARANNA
  • NAKASHIMA FUMIO
  • SAKAMOTO NOBUYUKI
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
ライセンスをご希望の方、特許の内容に興味を持たれた方は、下記「問合せ先」まで直接お問い合わせください。

PAGE TOP

close
close
close
close
close
close