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BIOFUEL PRODUCTION TECHNOLOGY USING MIXED-LIQUID, MIXED-SPECIES CULTURING 新技術説明会

外国特許コード F160008882
整理番号 (S2015-0747-N0)
掲載日 2016年10月25日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2016JP057691
国際公開番号 WO 2016140374
国際出願日 平成28年3月4日(2016.3.4)
国際公開日 平成28年9月9日(2016.9.9)
優先権データ
  • 特願2015-043817 (2015.3.5) JP
発明の名称 (英語) BIOFUEL PRODUCTION TECHNOLOGY USING MIXED-LIQUID, MIXED-SPECIES CULTURING 新技術説明会
発明の概要(英語) The purpose of the present invention is to provide a novel technology for producing biofuel, and, specifically, the present invention relates to a biofuel manufacturing method that includes coculturing of mixed microorganisms including photosynthetic microorganisms and non-photosynthetic microorganisms by using a nitrogen-source deprived medium.
従来技術、競合技術の概要(英語) BACKGROUND ART
Fossil fuel energy (natural gas, petroleum, coal or the like) and in view of the current depends on Japan, future, and of the fuel by itself can be produced by a technique is important. Therefore, the water and carbon dioxide is fixed by photosynthesis, can be an organic substance such as glucose to produce biofuel using photosynthetic organisms to produce very inside Japan attempt has attracted attention. Including plasfocjanin (prokaryotic photosynthetic microorganisms) photosynthetic microorganisms, which belong to the microalgae in the broader sense, photosynthesis ability as compared to that of the plant is one hundred times to several tens are said to be. In addition, in the case of using the microalgae biofuel production, such as rhizomes or other cultivated plant as compared to after the unnecessary waste does not arise from the fact that, as used in the next generation biofuel production has been regarded as promising organism. However, the biofuel is, as a raw material of the biodiesel or fatty acid triglyceride (triacylglycerol, TAG), roughly divided into such as a hydrocarbon. 2 The former one oxygen molecule (O) whose chemical structure has a, hydrocarbon and hydrogen (H) carbon (C) only and, as a fuel to the engine and the environmentally friendly been favored in petroleum industry. And hydrocarbon fuel having a carbon number of the double bond connecting them by the number and position, defined its properties as a fuel. A chain contain double bonds among the saturated hydrocarbons are, alkane (Cn H2n+2) referred to, to the short-chain alkane is 1-20 carbon atoms, various introduction we serves as a fuel. In particular carbon number alkane is 12-17 or 11-17, which are liquids at standard (at normal temperature and pressure) and, as a fuel light oil positioned corresponding to the jet fuel. Up to now of microalgae, some species of cyanobacteria in natural algae, having a carbon number of 15 (pentadecane, C15 H32) or 17 (heptadecanoic, c17 H36) alkane producing strain (non-patent document 1) are known. Group is the present inventors, it was modified by genetic manipulation of natural algae cyanobacteria, per cell dry weight up to about 50-60% mutants are successfully acquired (Patent Document 1). Not limited to this natural algae and in genetic recombination of the microalgae for biofuel production using algae has been attempted. Those in the prior art, isolated was purified algal cell in a culture medium were sterilized in a sterile or aseptically by culturing while approaching, the bio fuel into and out of the algae have been produced. In addition, as can be seen in bioethanol production of articles, produced from the plant after collection of the glucose, to obtain the target 2 by yeast fermentation production stage compared with the technology, is advantageous in using photosynthetic microorganisms, from glucose as described above for the purpose of the organic material (bio-fuel and the like) stage 1 (one within the bioreactor were cultured at the same time) can be produced according to this invention. On the other hand, useful substance production by co-culture as an example of the conventional method, , bacteria and yeast famentasu co-culture system for methyl halides (Patent Document 2), the culture medium wherein the green astika karius bo trio lactococcus, eccentric bird kas strain was added and the co-culture system to promote the proliferation of green algae (Patent Document 3), hydrolyzed from a biomass feedstock in a system for ethanol fuel Clostridial, further comprising phyto fell men chest of drawers or other fine cells by co-culture method (patent document 4) and the like.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • IBARAKI UNIVERSITY
  • 発明者(英語)
  • ASAYAMA MUNEHIKO
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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